Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 호정규 | - |
dc.date.accessioned | 2019-09-16T01:09:31Z | - |
dc.date.available | 2019-09-16T01:09:31Z | - |
dc.date.issued | 2019-03 | - |
dc.identifier.citation | DIGESTIVE DISEASES AND SCIENCES, v. 64, NO 3, Page. 781-791 | en_US |
dc.identifier.issn | 0163-2116 | - |
dc.identifier.issn | 1573-2568 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs10620-018-5363-2 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/110428 | - |
dc.description.abstract | BackgroundInjecting MSCs via blood vessel is most commonly used method, which has a major drawback of safety. The aim of our study was to evaluate efficacy using scaffold-loaded MSCs in acute liver failure model.MethodAcute liver failure was induced in mice using thioacetamide (TAA) (200mg/kg, i.p) once a day for two consecutive days. The animals were divided in four acute liver failure groups: (1) TAA; (2) empty scaffold; (3) MSCs injected through tail vein; (4) MSC+Scaffold, scaffold loaded with MSCs, to evaluate the mortality and changes in liver function. Polylactic-co-glycolic acid scaffold alone and loaded with human MSCs was implanted on mice dorsum.ResultsTAA dose was titrated until one-third mortality rate was achieved. TAA (200mg/kg) once daily for two consecutive days was injected to establish the acute liver failure model. The mortality of TAA and scaffold groups was 55.9% and 63.2%, respectively. Although, mortality of MSC-TV group decreased 14.7% as compared to TAA group (p=0.200), MSC+Scaffold group had the lowest mortality (31.4%) (p=0.013). Cells implanted in PLGA biomaterial were survived until 3weeks, and their function was increased. Area of hepatic inflammation and necrosis was significantly reduced in MSC-TV and MSC+Scaffold groups; but there was no difference between the two groups. Gene expressions related to inflammation were significantly decreased in MSC-TV and MSC+Scaffold groups compared to TAA group. In MSC+Scaffold group, no migration of stem cells to liver tissue was observed. Although, not all cells in scaffold were stained, some of them were differentiated into hepatocyte-like cells which stained positive for PAS and CYP2E1 antibody.ConclusionScaffold loaded with MSCs showed protective effects via paracrine signaling on acute liver failure model. | en_US |
dc.description.sponsorship | This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2015R1D1A1A02061717). | en_US |
dc.language.iso | en | en_US |
dc.publisher | SPRINGER | en_US |
dc.subject | Acute liver failure | en_US |
dc.subject | Mesenchymal stem cell | en_US |
dc.subject | Scaffold | en_US |
dc.subject | Paracrine effect | en_US |
dc.title | Effect of Stem Cell Treatment on Acute Liver Failure Model Using Scaffold | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 64 | - |
dc.identifier.doi | 10.1007/s10620-018-5363-2 | - |
dc.relation.page | 781-791 | - |
dc.relation.journal | DIGESTIVE DISEASES AND SCIENCES | - |
dc.contributor.googleauthor | Kang, Hyeon Tae | - |
dc.contributor.googleauthor | Jun, Dae Won | - |
dc.contributor.googleauthor | Jang, Kiseok | - |
dc.contributor.googleauthor | Hoh, Jeong-Kyu | - |
dc.contributor.googleauthor | Lee, Jai Sun | - |
dc.contributor.googleauthor | Saeed, Waqar Khalid | - |
dc.contributor.googleauthor | Chae, Yeon Ji | - |
dc.contributor.googleauthor | Lee, Jin Ho | - |
dc.relation.code | 2019000811 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | hohjk | - |
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