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dc.contributor.author이은규-
dc.date.accessioned2019-09-09T06:03:09Z-
dc.date.available2019-09-09T06:03:09Z-
dc.date.issued2005-04-
dc.identifier.citationPROCESS BIOCHEMISTRY, v. 40, No. 5, Page. 1967-1972en_US
dc.identifier.issn1359-5113-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0032959204003425-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/110406-
dc.description.abstractLipoprotein kringle "LK68" is a polypeptide of a modified angiostatin consisting of three kringle structures that might be clinically useful as potential cancer therapeutics. It can be produced by overexpressing it as an inclusion body in recombinant Escherichia coli. In this study, solid-phase refolding processes using a packed bed (PBA) and expanded bed adsorption (EBA) column were carried out to compare their refolding yields with that of the conventional, solution-phase refolding process. For the solution-phase and the PBA-mediated processes employing Q-Sepharose, washed inclusion body was used as the starting material, whereas both washed inclusion body and E. coli homogenate were used for the EBA-mediated process employing STREAMLINE DEAE. On a per unit mass of wet cell basis, the EBA- and PBA-mediated process showed about 4.3- and 1.7-fold higher yields, respectively, than the solution-phase refolding method. The solid-phase refolded LK68 demonstrated the same lysine-binding bioactivity and the retention time in the RP- and SEC-HPLC as those of the native protein. (c) 2004 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipThis research was financially supported by Green Cross Biotech Corp., YongIn, Korea, and the Center for Advanced Bioseparation Technology at Inha University, Incheon, Korea.en_US
dc.language.isoen_USen_US
dc.publisherELSEVIER SCI LTDen_US
dc.subjectinclusion bodyen_US
dc.subjectsolid-phase refoldingen_US
dc.subjectrenaturationen_US
dc.subjectangiostatinen_US
dc.subjectexpanded bed adsorption chromatographyen_US
dc.titleSolid-phase refolding of inclusion body protein in a packed and expanded bed adsorption chromatographyen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.procbio.2004.07.012-
dc.relation.journalPROCESS BIOCHEMISTRY-
dc.contributor.googleauthorChoi, Won Chan-
dc.contributor.googleauthorKim, Min Young-
dc.contributor.googleauthorSuh, Chang Woo-
dc.contributor.googleauthorLee, Eun Kyu-
dc.relation.code2009207888-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF ENGINEERING SCIENCES[E]-
dc.sector.departmentDEPARTMENT OF BIONANO ENGINEERING-
dc.identifier.pideklee-
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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