Isolation of Salmonella enterica subspecies enterica serovar Paratyphi B dT(+), or Salmonella Java, from Indonesia and alteration of the d-tartrate fermentation phenotype by disrupting the ORF STM 3356
- Title
- Isolation of Salmonella enterica subspecies enterica serovar Paratyphi B dT(+), or Salmonella Java, from Indonesia and alteration of the d-tartrate fermentation phenotype by disrupting the ORF STM 3356
- Author
- 김동욱
- Keywords
- MDR; multidrug-resistant; MLST; multilocus sequence typing; SGI1; Salmonella genomic island 1
- Issue Date
- 2006-12
- Publisher
- SOC GENERAL MICROBIOLOGY
- Citation
- JOURNAL OF MEDICAL MICROBIOLOGY, v. 55, No. 12, Page. 1661-1665
- Abstract
- Salmonella enterica subspecies enterica serovar Paratyphi B [O1,4,(5),12: Hb: 1,2] can cause either an enteric fever (paratyphoid fever) or self-limiting gastroenteritis in humans. The d-tartrate non-fermenting variant S. enterica subsp. enterica serovar Paratyphi B dT(-) (S. Paratyphi B) is the causative agent of paratyphoid fever, and the d-tartrate fermenting variant S. enterica subsp. enterica serovar Paratyphi B dT(+) (S. Paratyphi B dT(+); formerly called Salmonella Java) causes gastroenteritis. S. Java is currently recognized as an emerging problem worldwide. Twelve dT(+) S. Java isolates were collected in Indonesia between 2000 and 2002. One-third of them contained Salmonella genomic island 1 (SGI1), which gives the multidrug-resistant phenotype to the bacteria. In this study, a PCR-based method to detect a single nucleotide difference responsible for the inability to ferment of-tartrate, reported elsewhere, was validated. The of-tartrate fermenting phenotype of S. Java was converted to the non-fermenting phenotype by the disruption of the ORF STM 3356, and the d-tartrate non-fermenting phenotype of the ORF STM 3356-disrupted strain and the dT(-) reference strain was changed to the dT(+) phenotype by complementing ORF STM 3356 in trans. The results show that the dT(+) phenotype requires a functional product encoded by STM 3356, and support the use of the PCR-based discrimination method for S. Paratyphi B and S. Java as the standard differentiation method.
- URI
- https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.46792-0https://repository.hanyang.ac.kr/handle/20.500.11754/110033
- ISSN
- 0022-2615; 1473-5644
- DOI
- 10.1099/jmm.0.46792-0
- Appears in Collections:
- COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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