Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이영한 | - |
dc.date.accessioned | 2019-08-27T06:45:39Z | - |
dc.date.available | 2019-08-27T06:45:39Z | - |
dc.date.issued | 2007-04 | - |
dc.identifier.citation | JOURNAL OF NEUROSCIENCE METHODS, v. 161, No. 2, Page. 199-204 | en_US |
dc.identifier.issn | 0165-0270 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0165027006005437 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/110002 | - |
dc.description.abstract | Neurofilaments (NFs) are heteropolymers composed of light (NF-L), middle (NF-M), and heavy (NF-H) subunits, present in most neurons. NF-L polymerizes on its own to provide a scaffold on which regular NFs form via the cross-bridging of NF-M or NF-H. To clarify the mechanism of regulation of NF-L self-assembly, we developed an assay using truncated mutant NF-L fused to glutathione-S transferase (GST). Western immunoblotting data show that the GST-fused head-rod domains of NF-L are necessary and sufficient for detecting assembled NF-L. The levels of self-assembled NF-L subunits detected using GST fusion proteins were consistent with those detected by electron microscopy and turbidity assay. Our results collectively imply that GST-fused head-rod domains of NF-L are critical tools for analyzing NF-L self-assembly in vitro. (C) 2006 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | We are grateful to Drs. Liem R.K.H. (Columbia University) and Hisanaga S. (Tokyo Metropolitan University) for providing rat cDNAs for NF-M. This work was supported by Grant number R01-2005-000-10728-0 from the Basic Research Program of the Korea Science & Engineering Foundation. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.subject | neurofilament light chain | en_US |
dc.subject | self-assembly assay | en_US |
dc.subject | GST pull-down | en_US |
dc.subject | turbidity | en_US |
dc.subject | electron microscopy | en_US |
dc.subject | Western blotting | en_US |
dc.subject | PC12 cells | en_US |
dc.subject | SW13 cells | en_US |
dc.title | In vitro assay of neurofilament light chain self-assembly using truncated mutants | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/j.jneumeth.2006.10.022 | - |
dc.relation.journal | JOURNAL OF NEUROSCIENCE METHODS | - |
dc.contributor.googleauthor | Kim, Sung-Kuk | - |
dc.contributor.googleauthor | Cho, Sang-Min | - |
dc.contributor.googleauthor | Lee, In-Bum | - |
dc.contributor.googleauthor | Lee, Young Han | - |
dc.contributor.googleauthor | Kang, Jung Hoon | - |
dc.contributor.googleauthor | Choi, Jang Hyun | - |
dc.contributor.googleauthor | Suh, Pann-Ghill | - |
dc.contributor.googleauthor | Chang, Jong-Soo | - |
dc.relation.code | 2009205511 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF SCIENCE & TECHNOLOGY[E] | - |
dc.sector.department | DIVISION OF MOLECULAR & LIFE SCIENCE | - |
dc.identifier.pid | younghan | - |
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