Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이영한 | - |
dc.date.accessioned | 2019-08-22T07:04:16Z | - |
dc.date.available | 2019-08-22T07:04:16Z | - |
dc.date.issued | 2006-12 | - |
dc.identifier.citation | EXPERIMENTAL AND MOLECULAR MEDICINE, v. 38, No. 6, Page. 677-685 | en_US |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.issn | 2092-6413 | - |
dc.identifier.uri | https://www.nature.com/articles/emm200680 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/108970 | - |
dc.description.abstract | The early growth response-1 gene (egr-1) encodes a zinc-finger transcription factor Egr-1 and is rapidly inducible by a variety of extracellular stimuli. Anisomycin (ANX), a protein synthesis inhibitor, stimulates mitogen-activated protein kinase (MAPK) pathways and thereby causes a rapid induction of immediate-early response genes. We found that anisomycin treatment of U87MG glioma cells resulted in a marked, time-dependent increase in levels of Egr-1 protein. The results of Northern blot analysis and reporter gene assay of egr-1 gene promoter (Pegr-1) activity indicate that the ANX-induced increase in Egr-1 occurs at the transcriptional level. Deletion of the serum response element (SRE) in the 5`-flanking region of egr-1 gene abolished ANX-induced Pegr-1 activity. ANX induced the phosphorylation of the ERK1/2, JNK, and p38 MAPKs in a time-dependent manner and also induced transactivation of Gal4-Elk-1, suggesting that Elk-1 is involved in SRE-mediated egr-1 transcription. Transient transfection of dominant-negative constructs of MAPK pathways blocked ANX-induced Pegr-1 activity. Furthermore, pretreatment with specific MAPK pathway inhibitors, including the MEK inhibitor U0126, the JNK inhibitor SP600125, and the p38 kinase inhibitor SB202190, completely inhibited ANX-inducible expression of Egr-1. Taken together, these results suggest that all three MAPK pathways play a crucial role in ANX-induced transcriptional activation of Pegr-1 through SRE-mediated transactivation of Elk-1. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY | en_US |
dc.subject | anisomycin | en_US |
dc.subject | cycloheximide | en_US |
dc.subject | early growth response protein 1 | en_US |
dc.subject | ets-domain protein Elk-1 | en_US |
dc.subject | mitogen-activated protein kinases | en_US |
dc.subject | serum response element | en_US |
dc.title | The translation inhibitor anisomycin induces Elk-1-mediated transcriptional activation of egr-1 through multiple mitogen-activated protein kinase pathways | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1038/emm.2006.80 | - |
dc.relation.journal | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.contributor.googleauthor | Shin, Soon Young | - |
dc.contributor.googleauthor | Lee, Joon Ho | - |
dc.contributor.googleauthor | Min, Byung Wook | - |
dc.contributor.googleauthor | Lee, Young Han | - |
dc.relation.code | 2009210380 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF SCIENCE & TECHNOLOGY[E] | - |
dc.sector.department | DIVISION OF MOLECULAR & LIFE SCIENCE | - |
dc.identifier.pid | younghan | - |
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