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Characteristics of a liposome immunoassay on a poly(methyl methacrylate) surface

Title
Characteristics of a liposome immunoassay on a poly(methyl methacrylate) surface
Author
이은규
Keywords
liposome immunoassay; immunoliposome; PMMA; microarray detection; signal enhancement
Issue Date
2007-12
Publisher
SPRINGER HEIDELBERG
Citation
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 389, No. 7-8, Page. 2251-2257
Abstract
Liposome immunoassay (LIA) is based on enzyme immunoassay (EIA) but the detection sensitivity could be significantly enhanced by using antibody-coupled immunoliposomes encapsulating HRP (horse radish peroxidase). Here, we applied LIA to non-porous poly(methyl methacrylate) (PMMA) and polystyrene (PS) surfaces to compare its detection sensitivity with that of EIA, using rabbit IgG (a ligand molecule) and anti-rabbit IgG antibody (a capture molecule) as the model system. LIA developed much stronger color signals than EIA, especially at a lower concentration range (< ca. 1 mu g mL(-1)). PMMA showed higher affinity toward rabbit IgG than the PS surface, and the anti-rabbit IgG antibody adsorbed on PMMA was more stable than that on PS. Furthermore, the effects of spot volume and antibody concentration on the signal density were analyzed. The signal density increased as the antibody concentration increased, but it was not significantly affected by the spot volume (2.5-20 mu L). In conclusion, LIA on PMMA as a solid support is a very useful, highly sensitive microarray detection system.
URI
https://link.springer.com/article/10.1007/s00216-007-1614-3https://repository.hanyang.ac.kr/handle/20.500.11754/107548
ISSN
1618-2642
DOI
10.1007/s00216-007-1614-3
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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