Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최준호 | - |
dc.date.accessioned | 2019-07-17T01:42:33Z | - |
dc.date.available | 2019-07-17T01:42:33Z | - |
dc.date.issued | 2019-02 | - |
dc.identifier.citation | CELL REPORTS, v. 26, NO 8, Page. 2126-2126 | en_US |
dc.identifier.issn | 2211-1247 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S221112471930138X?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/107487 | - |
dc.description.abstract | Exon junction complexes (EJCs) loaded onto spliced mRNAs during splicing serve as molecular markers for various post-transcriptional gene-regulatory processes, including nonsense-mediated mRNA decay (NMD). Although the composition and structure of EJCs are well characterized, the mechanism regulating EJC deposition remains unknown. Here we find that threonine 163 (T163) within the RNA-binding motif of eIF4A3 (a core EJC component) is phosphorylated by cyclin-dependent protein kinases 1 and 2 in a cell cycle-dependentmanner. T163 phosphorylation hinders binding of eIF4A3 to spliced mRNAs and other EJC components. Instead, it promotes association of eIF4A3 with CWC22, which guides eIF4A3 to an active spliceosome. These molecular events ensure the fidelity of specific deposition of the EJC similar to 20-24 nt up-stream of an exon-exon junction. Accordingly, NMD is affected by T163 phosphorylation. Collectively, our data provide evidence that T163 phosphorylation affects EJC formation and, consequently, NMD efficiency in a cell cycle-dependent manner. | en_US |
dc.description.sponsorship | We thank Niels Gehring for providing plasmid pCZ-CWC22 and Melisa Moore for pCN-MS2-Y14 and pCN-MS2-MAGOH. This work was supported by a National Research Foundation (NRF) of Korea grant funded by the Korean government (Ministry of Science, ICT, and Future Planning; NRF-2015R1A3A2033665 and NRF-2018R1A5A1024261), by a Korea University future research grant (K1720051), and by a Korea University grant, South Korea. I.R. and Y.S.W. were supported in part by the NRF funded by the Ministry of Education, Science and Technology, South Korea (NRF-2016R1D1A1B03933894 and NRF-2016R1A6A3A11933515, respectively). | en_US |
dc.language.iso | en | en_US |
dc.publisher | CELL PRESS | en_US |
dc.subject | EXON JUNCTION COMPLEX | en_US |
dc.subject | MESSENGER-RNA DECAY | en_US |
dc.subject | ENHANCES TRANSLATION | en_US |
dc.subject | CRYSTAL-STRUCTURE | en_US |
dc.subject | MAMMALIAN-CELLS | en_US |
dc.subject | PROTEIN | en_US |
dc.subject | REVEALS | en_US |
dc.subject | IDENTIFICATION | en_US |
dc.subject | CORE | en_US |
dc.subject | SEQ | en_US |
dc.title | eIF4A3 Phosphorylation by CDKs Affects NMD during the Cell Cycle | en_US |
dc.type | Article | en_US |
dc.relation.no | 8 | - |
dc.relation.volume | 26 | - |
dc.identifier.doi | 10.1016/j.celrep.2019.01.101 | - |
dc.relation.page | 2126-2126 | - |
dc.relation.journal | CELL REPORTS | - |
dc.contributor.googleauthor | Ryu, Incheol | - |
dc.contributor.googleauthor | Won, You-Sub | - |
dc.contributor.googleauthor | Ha, Hongseok | - |
dc.contributor.googleauthor | Kim, Eunjin | - |
dc.contributor.googleauthor | Park, Yeonkyoung | - |
dc.contributor.googleauthor | Kim, Min Kyung | - |
dc.contributor.googleauthor | Kwon, Do Hoon | - |
dc.contributor.googleauthor | Choe, Junho | - |
dc.contributor.googleauthor | Song, Hyun Kyu | - |
dc.contributor.googleauthor | Jung, Hosung | - |
dc.relation.code | 2019036884 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | jcho2711 | - |
dc.identifier.researcherID | E-3410-2019 | - |
dc.identifier.orcid | http://orcid.org/0000-0002-2365-3755 | - |
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