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Photolithographic Fabrication of Poly(Ethylene Glycol) Microstructures for Hydrogel-Based Microreactors and Spatially Addressed Microarrays

Title
Photolithographic Fabrication of Poly(Ethylene Glycol) Microstructures for Hydrogel-Based Microreactors and Spatially Addressed Microarrays
Author
이은규
Keywords
Micropatterning; hydrogel; photolithography; poly(ethylene glycol) diacrylate (PEG-DA); microreactor; microarray
Issue Date
2007-11
Publisher
한국미생물생명공학회
Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v. 17, No. 11, Page. 1826-1832
Abstract
We describe the fabrication of poly(ethylene glycol) diacrylate (PEG-DA) hydrogel microstructures with a high aspect ratio and the use of hydrogel microstructures containing the enzyme β-galactosidase (β-Gal) or glucose oxidase (GOx)/horseradish peroxidase (HRP) as biosensing components for the simultaneous detection of multiple analytes. The diameters of the hydrogel microstructures were almost the same at the top and at the bottom, indicating that no differential curing occurred through the thickness of the hydrogel microstructure. Using the hydrogel microstructures as microreactors, β-Gal or GOx/HRP was trapped in the hydrogel array, and the time-dependent fluorescence intensities of the hydrogel array were investigated to determine the dynamic uptake of substrates into the PEG-DA hydrogel. The time required to reach steady-state fluorescence by glucose diffusing into the hydrogel and its enzymatic reactions with GOx and HRP was half the time required for resorufin β-D-galactopyranoside (RGB) when used as the substrate for β-Gal. Spatially addressed hydrogel microarrays containing different enzymes were micropatterned for the simultaneous detection of multiple analytes, and glucose and RGB solutions were incubated as substrates. These results indicate that there was no cross-talk between the β-Galimmobilizing hydrogel micropatches and the GOx/HRPimmobilizing micropatches.
URI
http://kiss.kstudy.com/thesis/thesis-view.asp?key=2649432https://repository.hanyang.ac.kr/handle/20.500.11754/107234
ISSN
1017-7825; 1738-8872
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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