Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 정봉근 | - |
dc.date.accessioned | 2019-07-09T04:19:48Z | - |
dc.date.available | 2019-07-09T04:19:48Z | - |
dc.date.issued | 2007-10 | - |
dc.identifier.citation | BIOMEDICAL MICRODEVICES, v. 9, No. 5, Page. 627-635 | en_US |
dc.identifier.issn | 1387-2176 | - |
dc.identifier.issn | 1572-8781 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007/s10544-007-9051-9 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/107196 | - |
dc.description.abstract | We have developed a simple microfluidic device for generating stable concentration gradients in 2D and 3D environments. The device, termed the Ladder Chamber, uses a two-compartment diffusion system to generate steady state gradients across flow-free channels that connect the source and sink channels. To demonstrate the utility of the Ladder Chamber for cell migration, neutrophil chemotaxis was successfully observed in soluble chemoattractant (IL-8) gradient. The Ladder Chambers simple design and experimental implementation make it an attractive approach for investigating cell migration and other biological experiments in well-defined gradients in 2D surfaces as well as in 3D gels. | en_US |
dc.description.sponsorship | We thank Professor Steven S. George and Justin Mih for reagents and valuable discussions. We also thank Dr. Kapil Krishan for assistance with diffusion calculations, and Dr. Mike Papac for equipment. This research was supported by the Department of Defense (Grants no. DAMD17-03-1-0515 and no. DAMD17-03-1-0673). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | SPRINGER | en_US |
dc.subject | microfluidics | en_US |
dc.subject | concentration gradients | en_US |
dc.subject | ladder chamber | en_US |
dc.subject | cell migration | en_US |
dc.subject | neutrophil chemotaxis | en_US |
dc.title | Generation of stable concentration gradients in 2D and 3D environments using a microfluidic ladder chamber | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1007/s10544-007-9051-9 | - |
dc.relation.journal | BIOMEDICAL MICRODEVICES | - |
dc.contributor.googleauthor | Saadi, Wajeeh | - |
dc.contributor.googleauthor | Rhee, Seog Woo | - |
dc.contributor.googleauthor | Lin, Francis | - |
dc.contributor.googleauthor | Vahidi, Behrad | - |
dc.contributor.googleauthor | Chung, Bong Geun | - |
dc.contributor.googleauthor | Jeon, Noo Li | - |
dc.relation.code | 2007213034 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF ENGINEERING SCIENCES[E] | - |
dc.sector.department | DEPARTMENT OF BIONANO ENGINEERING | - |
dc.identifier.pid | bchung | - |
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