Generation of stable concentration gradients in 2D and 3D environments using a microfluidic ladder chamber
- Title
- Generation of stable concentration gradients in 2D and 3D environments using a microfluidic ladder chamber
- Author
- 정봉근
- Keywords
- microfluidics; concentration gradients; ladder chamber; cell migration; neutrophil chemotaxis
- Issue Date
- 2007-10
- Publisher
- SPRINGER
- Citation
- BIOMEDICAL MICRODEVICES, v. 9, No. 5, Page. 627-635
- Abstract
- We have developed a simple microfluidic device for generating stable concentration gradients in 2D and 3D environments. The device, termed the Ladder Chamber, uses a two-compartment diffusion system to generate steady state gradients across flow-free channels that connect the source and sink channels. To demonstrate the utility of the Ladder Chamber for cell migration, neutrophil chemotaxis was successfully observed in soluble chemoattractant (IL-8) gradient. The Ladder Chambers simple design and experimental implementation make it an attractive approach for investigating cell migration and other biological experiments in well-defined gradients in 2D surfaces as well as in 3D gels.
- URI
- https://link.springer.com/article/10.1007/s10544-007-9051-9https://repository.hanyang.ac.kr/handle/20.500.11754/107196
- ISSN
- 1387-2176; 1572-8781
- DOI
- 10.1007/s10544-007-9051-9
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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