Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이은규 | - |
dc.date.accessioned | 2019-06-24T02:41:28Z | - |
dc.date.available | 2019-06-24T02:41:28Z | - |
dc.date.issued | 2007-08 | - |
dc.identifier.citation | Biotechnology and Bioprocess Engineering, v. 12, No. 4, Page. 366-371 | en_US |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007%2FBF02931058 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/106790 | - |
dc.description.abstract | In this study, we used ITC (isothermal titration calorimetry) to quantitatively investigate the impacts of temperature and protein concentration on adsorption behavior on a solid surface, using BSA (bovine serum albumin) as a model protein, and alum (aluminum hydroxide) gel as an adsorbent. The zeta potential measurement for alum gel (0.25 mV at pH 9.3) revealed that its surface charge was not strong enough for electrostatic interaction. ITC analysis showed that the BSA-alum gel interaction was entropy-driven, suggesting that during adsorption, water molecules were expelled from the hydration layers of the alum gel and BSA. Therefore, the major mechanism for the BSA-alum gel interaction was hydrophobic interaction rather than electrostatic interaction. This biothermodynamic approach can be helpful not only to identify interaction mechanisms, but also to explore the optimum conditions for protein-adsorbent interactions. (c) KSBB. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | en_US |
dc.subject | ITC | en_US |
dc.subject | alum gel | en_US |
dc.subject | adjuvant | en_US |
dc.subject | adsorption | en_US |
dc.subject | biothermodynamics | en_US |
dc.subject | heat of adsorption | en_US |
dc.title | Biothermodynamic analysis of BSA adsorption to alum gel using isothermal titration calorimetry | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1007/BF02931058 | - |
dc.relation.journal | Biotechnology and Bioprocess Engineering | - |
dc.contributor.googleauthor | Kim, Ki Hyung | - |
dc.contributor.googleauthor | Lee, Eun Kyu | - |
dc.relation.code | 2007212003 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF ENGINEERING SCIENCES[E] | - |
dc.sector.department | DEPARTMENT OF BIONANO ENGINEERING | - |
dc.identifier.pid | eklee | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.