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dc.contributor.author이은규-
dc.date.accessioned2019-06-24T02:41:28Z-
dc.date.available2019-06-24T02:41:28Z-
dc.date.issued2007-08-
dc.identifier.citationBiotechnology and Bioprocess Engineering, v. 12, No. 4, Page. 366-371en_US
dc.identifier.issn1226-8372-
dc.identifier.urihttps://link.springer.com/article/10.1007%2FBF02931058-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/106790-
dc.description.abstractIn this study, we used ITC (isothermal titration calorimetry) to quantitatively investigate the impacts of temperature and protein concentration on adsorption behavior on a solid surface, using BSA (bovine serum albumin) as a model protein, and alum (aluminum hydroxide) gel as an adsorbent. The zeta potential measurement for alum gel (0.25 mV at pH 9.3) revealed that its surface charge was not strong enough for electrostatic interaction. ITC analysis showed that the BSA-alum gel interaction was entropy-driven, suggesting that during adsorption, water molecules were expelled from the hydration layers of the alum gel and BSA. Therefore, the major mechanism for the BSA-alum gel interaction was hydrophobic interaction rather than electrostatic interaction. This biothermodynamic approach can be helpful not only to identify interaction mechanisms, but also to explore the optimum conditions for protein-adsorbent interactions. (c) KSBB.en_US
dc.language.isoen_USen_US
dc.publisherKOREAN SOC BIOTECHNOLOGY & BIOENGINEERINGen_US
dc.subjectITCen_US
dc.subjectalum gelen_US
dc.subjectadjuvanten_US
dc.subjectadsorptionen_US
dc.subjectbiothermodynamicsen_US
dc.subjectheat of adsorptionen_US
dc.titleBiothermodynamic analysis of BSA adsorption to alum gel using isothermal titration calorimetryen_US
dc.typeArticleen_US
dc.identifier.doi10.1007/BF02931058-
dc.relation.journalBiotechnology and Bioprocess Engineering-
dc.contributor.googleauthorKim, Ki Hyung-
dc.contributor.googleauthorLee, Eun Kyu-
dc.relation.code2007212003-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF ENGINEERING SCIENCES[E]-
dc.sector.departmentDEPARTMENT OF BIONANO ENGINEERING-
dc.identifier.pideklee-
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COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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