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Biothermodynamic analysis of BSA adsorption to alum gel using isothermal titration calorimetry

Title
Biothermodynamic analysis of BSA adsorption to alum gel using isothermal titration calorimetry
Author
이은규
Keywords
ITC; alum gel; adjuvant; adsorption; biothermodynamics; heat of adsorption
Issue Date
2007-08
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Citation
Biotechnology and Bioprocess Engineering, v. 12, No. 4, Page. 366-371
Abstract
In this study, we used ITC (isothermal titration calorimetry) to quantitatively investigate the impacts of temperature and protein concentration on adsorption behavior on a solid surface, using BSA (bovine serum albumin) as a model protein, and alum (aluminum hydroxide) gel as an adsorbent. The zeta potential measurement for alum gel (0.25 mV at pH 9.3) revealed that its surface charge was not strong enough for electrostatic interaction. ITC analysis showed that the BSA-alum gel interaction was entropy-driven, suggesting that during adsorption, water molecules were expelled from the hydration layers of the alum gel and BSA. Therefore, the major mechanism for the BSA-alum gel interaction was hydrophobic interaction rather than electrostatic interaction. This biothermodynamic approach can be helpful not only to identify interaction mechanisms, but also to explore the optimum conditions for protein-adsorbent interactions. (c) KSBB.
URI
https://link.springer.com/article/10.1007%2FBF02931058https://repository.hanyang.ac.kr/handle/20.500.11754/106790
ISSN
1226-8372
DOI
10.1007/BF02931058
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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