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Surface-enhanced Raman spectroscopic-encoded beads for multiplex immunoassay

Title
Surface-enhanced Raman spectroscopic-encoded beads for multiplex immunoassay
Author
김종호
Keywords
PERFORMANCE LIQUID-CHROMATOGRAPHY; MONODISPERSE POLYMER BEADS; BUNDLE IMAGE COMPRESSION; SOLID-PHASE REACTIONS; COMBINATORIAL CHEMISTRY; SENSITIVE DETECTION; ORGANIC-SYNTHESIS; PACKING MATERIAL; SCATTERING; NANOPARTICLES
Issue Date
2007-03
Publisher
AMER CHEMICAL SOC
Citation
JOURNAL OF COMBINATORIAL CHEMISTRY, v. 9, No. 2, Page. 237-244
Abstract
A new type of encoded bead, which uses surface-enhanced Raman scattering (SERS), is described for multiplex immunoassays. Silver nanoparticles were embedded in sulfonated polystyrene (PS) beads via a polyol method, and they were used as SERS-active substrates. Raman-label organic compounds such as 4-methylbenzenethiol (4-MT), 2-naphthalenethiol (2-NT), and benzenethiol (BT) were then adsorbed onto the silver nanoparticles in the sulfonated PS bead. Although only three kinds of encoding have been demonstrated here, various combinations of these Raman-label organic compounds have the potential to give a large number of tags. The Raman-label-incorporated particles were then coated with a silica shell using tetraethoxyorthosilicate (TEOS) for chemical stability and biocompatibility. The resulting beads showed unique and intense Raman signals for the labeled organic compounds. We demonstrated that SERS-encoded beads could be used for multiplex detection with a model using streptavidin and p53. In our system, the binding event of target molecules and the type of ligand can be simultaneously recognized by Raman spectroscopy using a single laser-line excitation (514.5 nm).
URI
https://pubs.acs.org/doi/abs/10.1021/cc0600831https://repository.hanyang.ac.kr/handle/20.500.11754/106330
ISSN
1520-4766
DOI
10.1021/cc0600831
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > MATERIALS SCIENCE AND CHEMICAL ENGINEERING(재료화학공학과) > Articles
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