Advanced selection methodologies for DNAzymes in sensing and healthcare applications.
- Title
- Advanced selection methodologies for DNAzymes in sensing and healthcare applications.
- Author
- 김기현
- Keywords
- INHIBIT CELL-PROLIFERATION; HIGHLY SENSITIVE DETECTION; DNA-CLEAVING DNAZYME; IN-VITRO SELECTION; APTAMER SELECTION; RNA APTAMERS; CAPILLARY-ELECTROPHORESIS; ELECTROCHEMICAL SENSORS; ADENOSINE-TRIPHOSPHATE; CARBON NANOTUBES
- Issue Date
- 2019-03
- Publisher
- ELSEVIER SCIENCE LONDON
- Citation
- TRENDS IN BIOCHEMICAL SCIENCES, v. 44, NO 3, Page. 190-213
- Abstract
- DNAzymes have been widely explored owing to their excellent catalytic activity in a broad range of applications, notably in sensing and biomedical devices. These newly discovered applications have built high hopes for designing novel catalytic DNAzymes. However, the selection of efficient DNAzymes is a challenging process but one that is of crucial importance. Initially, systemic evolution of ligands by exponential enrichment (SELEX) was a labor-intensive and time-consuming process, but recent advances have accelerated the automated generation of DNAzyme molecules. This review summarizes recent advances in SELEX that improve the affinity and specificity of DNAzymes. The thriving generation of new DNAzymes is expected to open the door to several healthcare applications. Therefore, a significant portion of this review is dedicated to various biological applications of DNAzymes, such as sensing, therapeutics, and nanodevices. In addition, discussion is further extended to the barriers encountered for the real-life application of these DNAzymes to provide a foundation for future research.
- URI
- https://www.sciencedirect.com/science/article/pii/S0968000418302287?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/106033
- ISSN
- 0968-0004; 1362-4326
- DOI
- 10.1016/j.tibs.2018.11.001
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > CIVIL AND ENVIRONMENTAL ENGINEERING(건설환경공학과) > Articles
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