Simultaneous Determination of Bioactive Xanthone Glycosides and Norlignans from Ethanolic Extract of Anemarrhena asphodeloides by Liquid Chromatography
- Title
- Simultaneous Determination of Bioactive Xanthone Glycosides and Norlignans from Ethanolic Extract of Anemarrhena asphodeloides by Liquid Chromatography
- Author
- 유혜현
- Keywords
- TANDEM MASS-SPECTROMETRY; SWERTIA-MUSSOTII FRANCH; RAT PLASMA; CAPILLARY-ELECTROPHORESIS; ANTIDIABETIC ACTIVITY; ANTIOXIDANT ACTIVITY; ACTIVE CONSTITUENTS; MANGIFERIN; IDENTIFICATION; RHIZOMES
- Issue Date
- 2008-12
- Publisher
- AOAC INTERNATIONAL
- Citation
- JOURNAL OF AOAC INTERNATIONAL, v. 91, No. 6, Page. 1271-1277
- Abstract
- The rhizomes of Anemarrhena asphodeloides Bunge (Liliaceae) are prescribed as crude drugs in herbal medication for the treatment of various diseases such as diabetes, inflammation, and platelet aggregation inhibition. A simple, sensitive, and precise reversed-phase liquid chromatographic method was developed to study the quantitative determination of 5 bioactive compounds from these rhizomes, namely, neomangiferin, mangiferin, isomangiferin, nyasol, and methylnyasol. Chromatographic analysis was performed on Capcell Pak C-18 column (150 x 4.6 mm, 3 mu m) with a mobile phase consisting of acetonitrile, methanol, and 0.1% formic acid at a flow rate of 1.00 mL/min. Quantitation was performed using a UV-visible detector at 260 nm. The method for the determination of reported medicinal agents was accurate and reproducible. Excellent linear behavior was observed over the investigated concentration range of 2.5-100.0 mu g/mL for neomangiferin; 1.5-60.0 mu g/mL for mangiferin; 0.5-20.0 mu g/mL for nyasol; and 0.2-20.0 mu g/mL for methylnyasol; correlation coefficient > 0.99. The intraday and interday precision over the concentration range of compounds was < 6.6% (relative standard deviation) and accuracy was between 94.9 and 109.3%. This method can be successfully applied for the analysis of medicinal compounds from the ethanolic extract of A. asphodeloides Bunge.
- URI
- https://www.ingentaconnect.com/content/aoac/jaoac/2008/00000091/00000006/art00004https://repository.hanyang.ac.kr/handle/20.500.11754/105275
- ISSN
- 1060-3271
- Appears in Collections:
- COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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