57 0

Full metadata record

DC FieldValueLanguage
dc.contributor.author이철훈-
dc.date.accessioned2019-05-20T05:58:24Z-
dc.date.available2019-05-20T05:58:24Z-
dc.date.issued2008-09-
dc.identifier.citationJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v. 18, No. 9, Page. 1584-1589en_US
dc.identifier.issn1017-7825-
dc.identifier.urihttp://www.jmb.or.kr/journal/view.html?book=Journal&tops=&start=0&scale=50&key=all&key_word=&Vol=18&Num=9&PG=&year1=&year2=&sort=publish_Date+desc&aut_box=Y&sub_box=Y&sos_box=&key_box=&pub_box=Y&abs_box=&mod=vol&mnum=311&multi%5B%5D=313&multi%5B%5D=316&multi%5B%5D=309&multi%5B%5D=314&multi%5B%5D=321&multi%5B%5D=317&multi%5B%5D=315&multi%5B%5D=312&multi%5B%5D=2685&multi%5B%5D=325&multi%5B%5D=310&multi%5B%5D=323&multi%5B%5D=318&multi%5B%5D=319&multi%5B%5D=324&multi%5B%5D=311&multi%5B%5D=326&multi%5B%5D=327&multi%5B%5D=322&multi%5B%5D=320-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/104843-
dc.description.abstractAlthough resistance of Helicobacter pylori to clarithromycin is a major cause of failure of eradication therapies, little information is available regarding gene mutations of clarithromycin-resistant primary and secondary H. pylori isolates in Korea. In the present study, we examined gene mutations of H. pylori 23S rRNA responsible for resistance to clarithromycin. DNA sequences of the 23S rRNA gene in 21 primary clarithromycin-resistant and 64 secondary clarithromycin-resistant strains were determined by PCR amplification and nucleotide sequence analyses. Two mutations of the 23S rRNA gene, A2143G and T2182C, were observed in primary clarithromycin-resistant isolates. In secondary isolates, dual mutation of A2143G+T2182C was frequently observed. In addition, A2143G+T2182C+ T2190C, A2143G+T2182C+C2195T, and A2143G+T2182C +A2223G were observed in secondary isolates. Furthermore, macrolide binding was tested on purified ribosomes isolated from T2182C or A2143C mutant strains with [C-14]erythromycin. Erythromycin binding increased in a dose-dependent manner for the susceptible strain but not for the mutant strains. These results indicate that secondary isolates show a greater variety of 23S rRNA gene mutation types than primary isolates, and triple mutations of secondary isolates are associated with A2143G+T2182C in H. pylori isolated from Korean patients.en_US
dc.description.sponsorshipWe thank Dr. SLing-il Cho for statistical analysis, and Mi-Soon Kim and Han-Jin Lee for their excellent technical help. We also thank Abbott Laboratories (Abbott Park, IL, USA) for gifts of clarithromycin. This study was supported by a grant of Seoul R&BD Program and a grant of the Korea Health 21 R&D project Ministry of Health & Welfare, Republic of Korea (02-PJ3-PG6-EV08-0001).en_US
dc.language.isoen_USen_US
dc.publisherKOREAN SOC MICROBIOLOGY & BIOTECHNOLOGYen_US
dc.subjectClarithromycinen_US
dc.subjectgene mutationsen_US
dc.subjectHelicobacter pylorien_US
dc.subjectprimary isolatesen_US
dc.subjectresistanceen_US
dc.subjectsecondary isolatesen_US
dc.titleGene Mutations of 23S rRNA Associated with Clarithromycin Resistance in Helicobacter pylori Strains Isolated from Korean Patientsen_US
dc.typeArticleen_US
dc.relation.journalJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY-
dc.contributor.googleauthorKim, Jung Mogg-
dc.contributor.googleauthorKim, Joo Sung-
dc.contributor.googleauthorKim, Nayoung-
dc.contributor.googleauthorKim, Yeoung-Jeon-
dc.contributor.googleauthorKim, In Young-
dc.contributor.googleauthorChee, Young Joon-
dc.contributor.googleauthorLee, Chul-Hoon-
dc.contributor.googleauthorJung, Hyun Chae-
dc.relation.code2009216838-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF PHARMACY[E]-
dc.sector.departmentDEPARTMENT OF PHARMACY-
dc.identifier.pidchhlee-
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE