125 0

Full metadata record

DC FieldValueLanguage
dc.contributor.author이성환-
dc.date.accessioned2019-05-20T05:25:15Z-
dc.date.available2019-05-20T05:25:15Z-
dc.date.issued2008-08-
dc.identifier.citationELECTROPHORESIS, v. 29, No. 16, Page. 3466-3476en_US
dc.identifier.issn0173-0835-
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/abs/10.1002/elps.200800139-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/104780-
dc.description.abstractMicrofluidic-based microchips have become the focus of research interest for immunoassays and biomarker diagnostics. This is due to their aptitude for high-throughput processing, small sample volume, and short analysis times. In this paper, we describe the development of a microchip-based multiplex electro-immunosensing system for simultaneous detection of cancer biomarkers using gold nanoparticles and silver enhancer. Our microchip is composed of biocompatible poly(PDMS) and glass substrates. To fix the antibody-immobilized microbeads, we used pillar-type microfilters within a reaction chamber. An immunogold silver staining (IGSS) method was used to amplify the electrical signal that corresponded to the immunecomplex. To demonstrate this approach, we simultaneously assayed three cancer biomarkers, alpha -fetoprotein (AFP), carcinoembryonic antigen (CEA), and prostate-specific antigen (PSA) on the microchip. The electrical signal generated from the result of the immunoreaction was measured and monitored by a PC-based system. The overall assay time was reduced from 3-8 h to about 55 min when compared to conventional immunoassays. The working range of the proposed microchip was from 10-3 to 10-1 mu g/mL of the target antigen.en_US
dc.description.sponsorshipThis work was supported by grant no. R01-2003-000-10614-0 from Basic Research Program of the Korea Science and Engineering Foundation.en_US
dc.language.isoen_USen_US
dc.publisherWILEY-V C H VERLAG GMBHen_US
dc.subjectcancer biomarkeren_US
dc.subjectimmunogold silver stainingen_US
dc.subjectmicrobead-based immunoassayen_US
dc.subjectmicrofilter-based immobilizationen_US
dc.subjectmultiplex electro-immunosensing microchipen_US
dc.titleMicrochip -Based Multiplex Electro-Immunosensing System for the Detection of Cancer Biomarkersen_US
dc.typeArticleen_US
dc.identifier.doi10.1002/elps.200800139-
dc.relation.journalELECTROPHORESIS-
dc.contributor.googleauthorKo, Yong-Jun-
dc.contributor.googleauthorMaeng, Joon-Ho-
dc.contributor.googleauthorAhn, Yoomin-
dc.contributor.googleauthorHwang, Seung Yong-
dc.contributor.googleauthorCho, Nahm-Gyoo-
dc.contributor.googleauthorLee, Seoung-Hwan-
dc.relation.code2008202797-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF ENGINEERING SCIENCES[E]-
dc.sector.departmentDEPARTMENT OF MECHANICAL ENGINEERING-
dc.identifier.pidsunglee-
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > MECHANICAL ENGINEERING(기계공학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE