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Microchip -Based Multiplex Electro-Immunosensing System for the Detection of Cancer Biomarkers

Title
Microchip -Based Multiplex Electro-Immunosensing System for the Detection of Cancer Biomarkers
Author
이성환
Keywords
cancer biomarker; immunogold silver staining; microbead-based immunoassay; microfilter-based immobilization; multiplex electro-immunosensing microchip
Issue Date
2008-08
Publisher
WILEY-V C H VERLAG GMBH
Citation
ELECTROPHORESIS, v. 29, No. 16, Page. 3466-3476
Abstract
Microfluidic-based microchips have become the focus of research interest for immunoassays and biomarker diagnostics. This is due to their aptitude for high-throughput processing, small sample volume, and short analysis times. In this paper, we describe the development of a microchip-based multiplex electro-immunosensing system for simultaneous detection of cancer biomarkers using gold nanoparticles and silver enhancer. Our microchip is composed of biocompatible poly(PDMS) and glass substrates. To fix the antibody-immobilized microbeads, we used pillar-type microfilters within a reaction chamber. An immunogold silver staining (IGSS) method was used to amplify the electrical signal that corresponded to the immunecomplex. To demonstrate this approach, we simultaneously assayed three cancer biomarkers, alpha -fetoprotein (AFP), carcinoembryonic antigen (CEA), and prostate-specific antigen (PSA) on the microchip. The electrical signal generated from the result of the immunoreaction was measured and monitored by a PC-based system. The overall assay time was reduced from 3-8 h to about 55 min when compared to conventional immunoassays. The working range of the proposed microchip was from 10-3 to 10-1 mu g/mL of the target antigen.
URI
https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.200800139https://repository.hanyang.ac.kr/handle/20.500.11754/104780
ISSN
0173-0835
DOI
10.1002/elps.200800139
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > MECHANICAL ENGINEERING(기계공학과) > Articles
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