Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 유혜현 | - |
dc.date.accessioned | 2019-05-13T01:43:53Z | - |
dc.date.available | 2019-05-13T01:43:53Z | - |
dc.date.issued | 2009-04 | - |
dc.identifier.citation | JOURNAL OF AOAC INTERNATIONAL, v. 92, No. 2, Page. 375-381 | en_US |
dc.identifier.issn | 1060-3271 | - |
dc.identifier.uri | https://www.ingentaconnect.com/content/aoac/jaoac/2009/00000092/00000002/art00002 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/103918 | - |
dc.description.abstract | This paper describes a simple, rapid, and validated reversed-phase high-performance liquid chromatographic method developed for the determination of 4 major bioactive constituents, namely, chlorogenic acid, ferulic acid, senkyunolide A, and Z-ligustilide in Rhizoma Cnidii extract. A Capcell Pak C(18) chromatographic column (150 x 4.6 mm, 3 mu m) was used with mobile phases consisting of 0.1% formic acid, acetonitrile, and methanol at a flow rate of 0.8 mL/min and UV detection at 285 nm. Comprehensive validation of the method included evaluation of linearity, repeatability, recovery, and stability. Excellent linear behavior (r(2) >0.99) was observed over the concentration range of 2-100 mu g/mL for the compounds under investigation. Repeatability and accuracy were evaluated by intra- and interday assays; the relative standard deviation (RSD) values were <= 5.37% and accuracies ranged from 97.1 to 104.9%. Recoveries of the compounds ranged from 94.2 to 104.2% with RSD values of <= 9.50%. The developed method was successfully applied to the analysis of ethanolic extracts of Rhizoma Cnidii samples. As a result, the concentrations of chlorogenic acid, ferulic acid, Z-ligustilide, and senkyunolide A were determined to be 0.84-5.35, 0.45-1.65, 0.74-4.39, and 0.32-1.14 mg/g herb, respectively. Thus, the developed method was found to be accurate and reproducible and is considered suitable for the qualitative and quantitative analysis of Rhizoma Cnidii for bioactive compounds. | en_US |
dc.description.sponsorship | This work was supported in part by the Seoul Research and Business Development Program and in part by the Korea Institute of Science and Technology. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | AOAC INTERNATIONAL | en_US |
dc.subject | UMBELLIFEROUS MEDICINAL-PLANTS | en_US |
dc.subject | LIGUSTICUM-CHUANXIONG | en_US |
dc.subject | OFFICINALE MAKINO | en_US |
dc.subject | MASS-SPECTROMETRY | en_US |
dc.subject | ANGELICA-SINENSIS | en_US |
dc.subject | ESSENTIAL OIL | en_US |
dc.subject | FERULIC ACID | en_US |
dc.subject | CONSTITUENTS | en_US |
dc.subject | COMPONENTS | en_US |
dc.subject | CELLS | en_US |
dc.title | Simultaneous Determination of Phenolic Acids and Phthalide Compounds by Liquid Chromatography for Quality Assessment of Rhizoma Cnidii | en_US |
dc.type | Article | en_US |
dc.relation.volume | 92 | - |
dc.relation.page | 375-381 | - |
dc.relation.journal | JOURNAL OF AOAC INTERNATIONAL | - |
dc.contributor.googleauthor | Islam, M. Nurul | - |
dc.contributor.googleauthor | Yoo, Hye Hyun | - |
dc.contributor.googleauthor | Sung, Chung-Ki | - |
dc.contributor.googleauthor | Dong, Mi-sook | - |
dc.contributor.googleauthor | Park, Young In | - |
dc.contributor.googleauthor | Jin, Changbae | - |
dc.contributor.googleauthor | Kim, Dong-Hyun | - |
dc.relation.code | 2009204643 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | yoohh | - |
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