Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 정봉근 | - |
dc.date.accessioned | 2019-05-13T00:21:51Z | - |
dc.date.available | 2019-05-13T00:21:51Z | - |
dc.date.issued | 2009-03 | - |
dc.identifier.citation | LAB ON A CHIP, v. 9, No. 6, Page. 761-767 | en_US |
dc.identifier.issn | 1473-0197 | - |
dc.identifier.uri | http://pubs.rsc.org/en/content/articlehtml/2009/lc/b815990d | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/103889 | - |
dc.description.abstract | The ability to rapidly generate concentration gradients of diffusible molecules has important applications in many chemical and biological studies. Here we established spatially and temporally controllable concentration gradients of molecules (i.e. proteins or toxins) in a portable microfluidic device in an easy and rapid manner. The formation of the concentration gradients was initiated by a passive-pump-induced forward flow and further optimized during an evaporation-induced backward flow. The centimeter-long gradients along the microfluidic channel were shown to be spatially and temporally controlled by the backward flow. The gradient profile was stabilized by stopping the flow. Computational simulations of this dynamic process illustrated the combined effects of convection and diffusion on the gradient generation, and fit well with the experimental data. To demonstrate the applications of this methodology, a stabilized concentration gradient of a cardiac toxin, alphacypermethrin, along the microchannel was used to test the response of HL-1 cardiac cells in the micro-device, which correlated with toxicity data obtained from multi-well plates. The approach presented here may be useful for many biological and chemical processes that require rapid generation of long-range gradients in a portable microfluidic device. | en_US |
dc.description.sponsorship | This research has been funded by the US Army Engineer Research and Development Center, the Institute for Soldier Nanotechnology, NIH, the Coulter Foundation and the Draper Laboratory. We would like to thank Drs. Utkan Demirci, Young Song, Won Gu Lee, Edward Haeggstrom and Ms. Tracy Chang for the scientific and technical support. This research was supported in part by an appointment to the postgraduate research participation program at the US Army Engineer Research and Development Center, Construction Engineering Research Laboratory (ERDC-CERL), administered by the Oak Ridge Institute for Science and Education. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ROYAL SOC CHEMISTRY | en_US |
dc.subject | ROBUST MICROSCALE ASSAYS | en_US |
dc.subject | MANAGING EVAPORATION | en_US |
dc.subject | COMPLEX GRADIENTS | en_US |
dc.subject | CELL-CULTURE | en_US |
dc.subject | FLOW | en_US |
dc.subject | CHEMOTAXIS | en_US |
dc.subject | DISPERSION | en_US |
dc.subject | CHANNELS | en_US |
dc.subject | BIOLOGY | en_US |
dc.subject | ARRAYS | en_US |
dc.title | Rapid generation of spatially and temporally controllable long-range concentration gradients in a microfluidic device | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1039/b815990d | - |
dc.relation.journal | LAB ON A CHIP | - |
dc.contributor.googleauthor | Du, Yanan | - |
dc.contributor.googleauthor | Shim, Jaesool | - |
dc.contributor.googleauthor | Vidula, Mahesh | - |
dc.contributor.googleauthor | Hancock, Matthew J | - |
dc.contributor.googleauthor | Lo, Edward | - |
dc.contributor.googleauthor | Chung, Bong Geun | - |
dc.contributor.googleauthor | Borenstein, Jeffrey T | - |
dc.contributor.googleauthor | Khabiry, Masoud | - |
dc.contributor.googleauthor | Cropek, Donald M | - |
dc.contributor.googleauthor | Khademhosseini, Ali | - |
dc.relation.code | 2009214096 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF ENGINEERING SCIENCES[E] | - |
dc.sector.department | DEPARTMENT OF BIONANO ENGINEERING | - |
dc.identifier.pid | bchung | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.