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Rapid generation of spatially and temporally controllable long-range concentration gradients in a microfluidic device

Title
Rapid generation of spatially and temporally controllable long-range concentration gradients in a microfluidic device
Author
정봉근
Keywords
ROBUST MICROSCALE ASSAYS; MANAGING EVAPORATION; COMPLEX GRADIENTS; CELL-CULTURE; FLOW; CHEMOTAXIS; DISPERSION; CHANNELS; BIOLOGY; ARRAYS
Issue Date
2009-03
Publisher
ROYAL SOC CHEMISTRY
Citation
LAB ON A CHIP, v. 9, No. 6, Page. 761-767
Abstract
The ability to rapidly generate concentration gradients of diffusible molecules has important applications in many chemical and biological studies. Here we established spatially and temporally controllable concentration gradients of molecules (i.e. proteins or toxins) in a portable microfluidic device in an easy and rapid manner. The formation of the concentration gradients was initiated by a passive-pump-induced forward flow and further optimized during an evaporation-induced backward flow. The centimeter-long gradients along the microfluidic channel were shown to be spatially and temporally controlled by the backward flow. The gradient profile was stabilized by stopping the flow. Computational simulations of this dynamic process illustrated the combined effects of convection and diffusion on the gradient generation, and fit well with the experimental data. To demonstrate the applications of this methodology, a stabilized concentration gradient of a cardiac toxin, alphacypermethrin, along the microchannel was used to test the response of HL-1 cardiac cells in the micro-device, which correlated with toxicity data obtained from multi-well plates. The approach presented here may be useful for many biological and chemical processes that require rapid generation of long-range gradients in a portable microfluidic device.
URI
http://pubs.rsc.org/en/content/articlehtml/2009/lc/b815990dhttps://repository.hanyang.ac.kr/handle/20.500.11754/103889
ISSN
1473-0197
DOI
10.1039/b815990d
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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