Rapid identification of methylglyoxal trapping constituents from onion peels by pre-column incubation method
- Title
- Rapid identification of methylglyoxal trapping constituents from onion peels by pre-column incubation method
- Author
- 김철영
- Keywords
- Allium cepa L.; Methylglyoxal trapping; Pre-column incubation; Flavonoids
- Issue Date
- 2017-12
- Publisher
- Korean Society of Pharmacognosy
- Citation
- Natural Product Sciences, v. 23, No. 4, Page. 247-252
- Abstract
- The methylglyoxal (MGO) trapping constituents from onion (Allium cepa L.) peels were investigated using pre-column incubation of MGO and crude extract followed by HPLC analysis. The peak areas of MGO trapping compounds decreased, and their chemical structures were identified by HPLC-ESI/MS. Among major constituents in outer scale of onion, 2-(3,4-dihydroxybenzoyl)-2,4,6-trihydroxy-3(2H)-benzofuranone (2) was more effective MGO scavenger than quercetin (6) and its 4′-glucoside, spiraeoside (3). After 1 h incubation, compound 2 trapped over 90% MGO at a concentration of 0.5 mM under physiological conditions, but compounds 3 and 6 scavenged 45%, 16% MGO, respectively. HPLC-ESI/MS showed that compound 2 trapped two molecules of MGO to form a di-MGO adduct and compounds 3 and 6 captured one molecule of MGO to form mono-MGO adducts, and the positions 6 and 8 of the A ring of flavonoids were major active sites for trapping MGO.
- URI
- https://synapse.koreamed.org/DOIx.php?id=10.20307/nps.2017.23.4.247https://repository.hanyang.ac.kr/handle/20.500.11754/103805
- ISSN
- 1226-3907; 2288-9027
- DOI
- 10.20307/nps.2017.23.4.247
- Appears in Collections:
- COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML