A new coccolith modified electrode-based biosensor using a cognate pair of aptamers with sandwich-type binding
- Title
- A new coccolith modified electrode-based biosensor using a cognate pair of aptamers with sandwich-type binding
- Author
- 진언선
- Keywords
- A cognate pair of aptamers; Sandwich-type binding; Electrochemical biosensor; Coccolith; Chronoamperometry; Type 2 diabetes
- Issue Date
- 2019-01
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Citation
- BIOSENSORS & BIOELECTRONICS, v. 123, Page. 160-166
- Abstract
- In this study, we report a cognate pair of the aptamer-based sandwich-type electrochemical biosensor for type 2 diabetes biomarker (Vaspin) using coccolith modified electrodeposited on the screen-printed gold electrode (CME-SPGE). The coccolith derived from E. huxleyi used in this study were known to be highly-structured microparticles with many nano-sized pores. The CME-SPGE was successfully fabricated by drop-casting coccoliths, followed by Au sputtering and electrodeposition of Au. On this CME-SPGE electrode, the sandwich-type electrochemical aptasensor was fabricated by using a cognate pair of aptamers. The morphological, electrochemical characteristics and the performances of both the CME-SPGE and the completely fabricated sandwich type aptasensor were investigated by SEM, EDAX, cyclic voltammetry, and chronoamperometry. Due to the synergic effect of a cognate pair of aptamers on CME-SPGE, this newly developed sandwich-type electrochemical biosensor for Vaspin showed high specificity, and good sensitivity with a limit of detection (LOD) of 298 pM, along with more widen the linear range. To the best of our knowledge, this is the first report about the use of a coccolith modified electrode with a cognate pair aptamer resulting in sandwich-type binding in an electrochemical biosensor. With the advantages of using highly-structured biomineral microparticles and a cognate pair of aptamers, this new study may pave the innovative way to design a novel sandwich-type electrochemical aptasensor platform.
- URI
- https://www.sciencedirect.com/science/article/pii/S095656631830616X?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/103340
- ISSN
- 0956-5663; 1873-4235
- DOI
- 10.1016/j.bios.2018.08.021
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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