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A Highly sensitive electrochemical immunosensor for zearalenone using screen-printed disposable electrodes.

Title
A Highly sensitive electrochemical immunosensor for zearalenone using screen-printed disposable electrodes.
Author
김기현
Keywords
Zearalenone; Electrochemical immunosensor; BSA immobilization; Direct immobilization; Voltammetry
Issue Date
2019-01
Publisher
ELSEVIER SCIENCE SA
Citation
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 832, Page. 336-342
Abstract
Zearalenone (ZEN) is an important non-steroidal estrogenic mycotoxin produced by fusarium fungi species. A number of methods based on enzyme-linked immune assays (ELISA) and chromatographic approaches are available for the detection of ZEN. In this research, ZEN conjugated by bovine serum albumin (BSA) was immobilized on the surface of the screen-printed carbon electrode (SPCE) followed by the subsequent addition of anti-zearalenone monoclonal antibody (ZEN-MAb), alkaline phosphatase (ALP)-labeled secondary antibody, and 1-napthyl phosphate (1-NP) as a substrate. This immunosensor had good specificity, selectivity, reproducibility, and sensitivity for ZEN (LOD of 0.25 ng mL-5) when employed for quantification based on differential pulse voltammetry (DPV). This immunosensor yielded good recovery values in the range of 89-97% against real matrices (i.e., beer and wine samples) after spiking. This novel immunosensing principle can be extensively applied for the development of advanced sensing techniques for various analytes.
URI
https://www.sciencedirect.com/science/article/pii/S1572665718307240?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/102800
ISSN
1572-6657; 1873-2569
DOI
10.1016/j.jelechem.2018.10.058
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > CIVIL AND ENVIRONMENTAL ENGINEERING(건설환경공학과) > Articles
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