Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김은진 | - |
dc.date.accessioned | 2019-04-24T05:04:04Z | - |
dc.date.available | 2019-04-24T05:04:04Z | - |
dc.date.issued | 2016-09 | - |
dc.identifier.citation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v. 26, No. 12, Page. 2199-2205 | en_US |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.issn | 1738-8872 | - |
dc.identifier.uri | http://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1607.07022 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/102725 | - |
dc.description.abstract | Vibrio cholerae O1 serogroup Wave 3 El Tor strains are presently prevalent worldwide. The Wave 3 El Tor strains contain a TLC:RS1:CTX array on chromosome 1, and no element is integrated on chromosome 2. A multiplex PCR optimized to identify the TLC:RS1:CTX array of Wave 3 strains has been developed in this study. By using eight primers, the multiplex PCR can identify the characteristic CTX and RS1 array of Wave 3 strains from various arrays of strains belonging to other Waves. The four amplified DNA fragments of Wave 3 strains have been cloned in a vector, which could be used as a positive control for the multiplex PCR. This multiplex PCR and the positive control set could be useful tools for rapid recognition of Wave 3 El Tor strains. | en_US |
dc.description.sponsorship | This work was supported by the research fund of Hanyang University (HY-2012-P). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | en_US |
dc.subject | V. cholerae | en_US |
dc.subject | multiplex PCR | en_US |
dc.subject | GENETIC-CHARACTERIZATION | en_US |
dc.subject | VARIABLE NUMBER | en_US |
dc.subject | TANDEM REPEATS | en_US |
dc.subject | CTX PROPHAGES | en_US |
dc.subject | RS1 ELEMENT | en_US |
dc.subject | O1 | en_US |
dc.subject | KOLKATA | en_US |
dc.subject | DISSEMINATION | en_US |
dc.subject | OUTBREAK | en_US |
dc.subject | INDIA | en_US |
dc.title | Development of a multiplex PCR for discrimination of TLC:RS1:CTX array of Vibrio cholerae Wave 3 El Tor strains | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.4014/jmb.1607.07022 | - |
dc.relation.page | 2199-2205 | - |
dc.relation.journal | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.contributor.googleauthor | Kim, EJ | - |
dc.contributor.googleauthor | Yu, HJ | - |
dc.contributor.googleauthor | Nair, GB | - |
dc.contributor.googleauthor | Kim, DW | - |
dc.relation.code | 2016006799 | - |
dc.sector.campus | E | - |
dc.sector.daehak | RESEARCH INSTITUTE[E] | - |
dc.sector.department | INSTITUTE OF PHARMACEUTICAL SCIENCE AND TECHNOLOGY | - |
dc.identifier.pid | ejkim0816 | - |
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