Development of a multiplex PCR for discrimination of TLC:RS1:CTX array of Vibrio cholerae Wave 3 El Tor strains
- Title
- Development of a multiplex PCR for discrimination of TLC:RS1:CTX array of Vibrio cholerae Wave 3 El Tor strains
- Author
- 김은진
- Keywords
- V. cholerae; multiplex PCR; GENETIC-CHARACTERIZATION; VARIABLE NUMBER; TANDEM REPEATS; CTX PROPHAGES; RS1 ELEMENT; O1; KOLKATA; DISSEMINATION; OUTBREAK; INDIA
- Issue Date
- 2016-09
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v. 26, No. 12, Page. 2199-2205
- Abstract
- Vibrio cholerae O1 serogroup Wave 3 El Tor strains are presently prevalent worldwide. The Wave 3 El Tor strains contain a TLC:RS1:CTX array on chromosome 1, and no element is integrated on chromosome 2. A multiplex PCR optimized to identify the TLC:RS1:CTX array of Wave 3 strains has been developed in this study. By using eight primers, the multiplex PCR can identify the characteristic CTX and RS1 array of Wave 3 strains from various arrays of strains belonging to other Waves. The four amplified DNA fragments of Wave 3 strains have been cloned in a vector, which could be used as a positive control for the multiplex PCR. This multiplex PCR and the positive control set could be useful tools for rapid recognition of Wave 3 El Tor strains.
- URI
- http://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1607.07022https://repository.hanyang.ac.kr/handle/20.500.11754/102725
- ISSN
- 1017-7825; 1738-8872
- DOI
- 10.4014/jmb.1607.07022
- Appears in Collections:
- RESEARCH INSTITUTE[E](부설연구소) > INSTITUTE OF PHARMACEUTICAL SCIENCE AND TECHNOLOGY(약학기술연구소) > Articles
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