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Bioelectronic Nose Using Odorant Binding Protein-Derived Peptide and Carbon Nanotube Field-Effect Transistor for the Assessment of Salmonella Contamination in Food

Title
Bioelectronic Nose Using Odorant Binding Protein-Derived Peptide and Carbon Nanotube Field-Effect Transistor for the Assessment of Salmonella Contamination in Food
Author
이승환
Keywords
OLFACTORY BIOSENSOR; DROSOPHILA-MELANOGASTER; RECEPTOR; PERFORMANCE; IMMUNOASSAY; MIMICKING; MOLECULES; IMPEDANCE; PLATFORM; QUALITY
Issue Date
2016-12
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, V.88, No.23, Page.11283-11287
Abstract
Salmonella infection is the one of the major causes of food borne illnesses including fever, abdominal pain, diarrhea, and nausea. Thus, early detection of Salmonella contamination is important for our healthy life. Conventional detection methods for the food contamination have limitations in sensitivity and rapidity; thus, the early detection has been difficult. Herein, we developed a bioelectronic nose using a carbon nanotube (CNT) field-effect transistor (FET) functionalized with Drosophila odorant binding protein (OBP)-derived peptide for easy and rapid detection of Salmonella contamination in ham. 3-Methyl-1-butanol is known as a specific volatile organic compound, generated from the ham contaminated with Salmonella. We designed and synthesized the peptide based on the sequence of the Drosophila OBP, LUSH, which specifically binds to alcohols. The C-terminus of the synthetic peptide was modified with three phenylalanine residues and directly immobilized onto CNT channels using the pi-pi interaction. The p-type properties of FET were clearly maintained after the functionalization using the peptide. The biosensor detected 1 fM of 3-methyl-1-butanol with high selectivity and successfully assessed Salmonella contamination in ham. These results indicate that the bioelectronic nose can be used for the rapid detection of Salmonella contamination in food.
URI
https://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b03284https://repository.hanyang.ac.kr/handle/20.500.11754/102290
ISSN
0003-2700; 1520-6882
DOI
10.1021/acs.analchem.6b03284
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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