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dc.contributor.author김동욱-
dc.date.accessioned2019-04-15T02:03:37Z-
dc.date.available2019-04-15T02:03:37Z-
dc.date.issued2015-11-
dc.identifier.citationJOURNAL OF MEDICAL MICROBIOLOGY, v. 64, Page. 1335-1340en_US
dc.identifier.issn0022-2615-
dc.identifier.issn1473-5644-
dc.identifier.urihttp://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000164-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/101877-
dc.description.abstractTuberculosis (TB), an infectious disease caused by Mycobacterium tuberculosis complex (MTC), remains one of the leading causes of death in the world. In Korea, the current prevalence of multidrug-resistant TB (MDR-TB) poses a major problem. The most common method for diagnosing TB in developing countries is sputum smear microscopy; however, the sensitivity of this test is relatively low and it usually requires well-trained laboratory staff. Cultures of MTC require up to several weeks in sophisticated facilities, such as Biosafety Level 3. Effective diagnostic techniques are necessary to control TB. In Korea, we evaluated a loop-mediated isothermal amplification (LAMP) assay targeting the hspX gene (TB-hspX-LAMP) of MTC. For clinical evaluation, culture confirmation, smear microscopy and TB-hspX-LAMP were performed on 303 sputum specimens obtained from suspected TB patients in Korea. The sensitivity, specificity, positive predictive value and negative predictive value of TB-hspX-LAMP were 71.1, 98.8, 91.4 and 95.1 %, respectively, compared with TB culture, which is the gold standard for diagnosis of TB. In contrast, the comparable values of smear microscopy were 24.4, 98.1, 68.8 and 88.2 %, respectively. Therefore, we concluded that TB-hspX-LAMP was superior to the use of smear microscopy for the detection of MTC in sputum specimens in clinical settings in Korea.en_US
dc.description.sponsorshipThis work was supported by the National Research Foundation (NRF) of Korea (2015R1A2A2A01007297), and Japan Society for the Promotion of Science (JSPS) and the NRF under the Japan-Korea Basic Scientific Cooperation Program (NRF-2014K2A2A4001480). M. S. received research grant funding from Kaneka Co., Ltd. J. T. and S. M. are employees of Kaneka Co., Ltd.en_US
dc.language.isoen_USen_US
dc.publisherSOC GENERAL MICROBIOLOGYen_US
dc.subjectDIAGNOSISen_US
dc.subjectLAMPen_US
dc.subjectSAMPLESen_US
dc.subjectDNAen_US
dc.subjectMICROSCOPYen_US
dc.subjectSEQUENCEen_US
dc.subjectPCRen_US
dc.titleDetection of Mycobacterium tuberculosis complex in sputum specimens using a loop-mediated isothermal amplification assay in Koreaen_US
dc.typeArticleen_US
dc.relation.volume64-
dc.identifier.doi10.1099/jmm.0.000164-
dc.relation.page1335-1340-
dc.relation.journalJOURNAL OF MEDICAL MICROBIOLOGY-
dc.contributor.googleauthorMoon, Se Hoon-
dc.contributor.googleauthorKim, Eun Jin-
dc.contributor.googleauthorTomono, Jun-
dc.contributor.googleauthorMiyamoto, Shigehiko-
dc.contributor.googleauthorMitarai, Satoshi-
dc.contributor.googleauthorKim, Dong Wook-
dc.contributor.googleauthorSeki, Mitsuko-
dc.relation.code2015001984-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF PHARMACY[E]-
dc.sector.departmentDEPARTMENT OF PHARMACY-
dc.identifier.piddongwook-
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COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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