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dc.contributor.author윤채옥-
dc.date.accessioned2019-04-12T05:46:26Z-
dc.date.available2019-04-12T05:46:26Z-
dc.date.issued2016-12-
dc.identifier.citationONCOTARGET, v. 7, NO. 51, Page. 84965-84980en_US
dc.identifier.issn1949-2553-
dc.identifier.urihttp://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=13087&path[]=43960-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/101822-
dc.description.abstractTumor microenvironment is extremely immunosuppressive, preventing efficient induction of antitumor immunity. To overcome tumor-mediated immunosuppression and enhance the potency of immunogene therapy, oncolytic adenovirus (Ad) co-expressing interleukin (IL)-12 and vascular endothelial growth factor (VEGF)-specific short hairpin ribonucleic acid (shVEGF; RdB/IL12/shVEGF) was generated. Intratumoral injection of RdB/IL12/shVEGF induced a strong antitumor effect in an immune competent B16-F10 melanoma model. RdB/IL12/shVEGF restored immune surveillance function in tumor tissues and actively recruited immune cells by elevating the expression levels of IL-12 and interferon-γ. RdB/IL12/shVEGF efficiently suppressed expression of immunosuppressive VEGF, resulting in restoration of the antitumor immune response and prevention of thymic atrophy. In situ delivery of RdB/IL12/shVEGF to tumor tissues resulted in massive infiltration of differentiated CD4+ T cells, CD8+ T cells, natural killer cells, and dendritic cells to tissues surrounding the necrotic region of tumor. Furthermore, RdB/IL12/shVEGF induced a potent tumor-specific T helper type 1 immune response, implying that attenuation of the immunosuppressive environment mediated by downregulation of VEGF can significantly enhance immune stimulatory functions in the tumor milieu. Collectively, these findings indicate the potential of inducing and restoring potent antitumor immunity using intratumorally administered oncolytic Ad co-expressing IL-12 and shVEGF.en_US
dc.description.sponsorshipThis work was supported by grants from the National Research Foundation of Korea (2015R1A2A1A13027811; Dr. C-O Yun).en_US
dc.language.isoenen_US
dc.publisherIMPACT JOURNALS LLCen_US
dc.subjectcancer immunogene therapyen_US
dc.subjectoncolytic adenovirusen_US
dc.subjectinterleukin-12;en_US
dc.subjectvascular endothelial growth factoren_US
dc.subjectthymic atrophyen_US
dc.titleOncolytic adenovirus coexpressing interleukin-12 and shVEGF restores antitumor immune function adn enhances antitumor efficacy.en_US
dc.typeArticleen_US
dc.relation.no51-
dc.relation.volume7-
dc.identifier.doi10.18632/oncotarget.13087-
dc.relation.page84965-84980-
dc.relation.journalONCOTARGET-
dc.contributor.googleauthorAhn, Hyo Min-
dc.contributor.googleauthorHong, JinWoo-
dc.contributor.googleauthorYun, Chae-Ok-
dc.relation.code2016010107-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF ENGINEERING[S]-
dc.sector.departmentDEPARTMENT OF BIOENGINEERING-
dc.identifier.pidchaeok-
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COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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