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dc.contributor.author채영규-
dc.date.accessioned2019-03-25T07:11:12Z-
dc.date.available2019-03-25T07:11:12Z-
dc.date.issued2016-11-
dc.identifier.citationCURRENT MICROBIOLOGY, v. 73, NO.5, Page. 714-720en_US
dc.identifier.issn0343-8651-
dc.identifier.issn1432-0991-
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs00284-016-1120-y-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/101170-
dc.description.abstractCodY is a pleiotropic regulator commonly found in Gram-positive bacteria and regulates various biological processes during the stringent response in a nutrient-limiting environment. CodY also participates in virulence factor expression in many low G+C Gram-positive pathogens, as observed in Bacillus anthracis. However, the mechanism by which B. anthracis CodY regulates metabolism and virulence factors in response to environmental changes is unclear. Here, we attempted to identify the link between CodY and B. anthracis regulation with codY-deficient and codY-overexpressing mutants using high-throughput transcriptional analysis. Growth pattern analyses of codY mutants in both rich and minimal media showed defects in early cell proliferation, with opposite patterns in the early stationary phase: CodY overexpression prolonged bacterial growth, whereas deletion inhibited growth. RNA sequencing of codY-deficient B. anthracis showed both positive and negative changes in the gene expression of proteases and virulence factors as well as genes related to stringent response-related metabolism and biosynthetic processing. We also found that changes in codY expression could alter virulence gene expression of B. anthracis, suggesting modes of regulation in its virulence in a CodY concentration-dependent manner. Collectively, we conclude from these results that CodY can both positively and negatively regulate its regulon via direct and/or indirect approaches, and that its mode of regulation may be concentration dependent.en_US
dc.description.sponsorshipWe thank Dr. Konstantin Shatalin for providing an integration vector pKS1, and Dr. Abraham Sonenshein for anti-CodY antibody. This research was supported by Global Ph. D. Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2012-003722), NRF Grant funded by Korean Government (MSIP 2011-0030049) and Agency of Defense Development (UC13002320).en_US
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.subjectGRAM-POSITIVE BACTERIAen_US
dc.subjectTRANSCRIPTION INITIATIONen_US
dc.subjectSTREPTOCOCCUS-PYOGENESen_US
dc.subjectSTATIONARY-PHASEen_US
dc.subjectSUBTILIS CODYen_US
dc.subjectIN-VITROen_US
dc.subjectPATHOGENESISen_US
dc.subjectMECHANISMen_US
dc.subjectPPGPPen_US
dc.subjectCELLSen_US
dc.titleLate-Exponential Gene Expression in codY-Deficient Bacillus anthracis in a Host-Like Environmenten_US
dc.typeArticleen_US
dc.relation.no5-
dc.relation.volume73-
dc.identifier.doi10.1007/s00284-016-1120-y-
dc.relation.page714-720-
dc.relation.journalCURRENT MICROBIOLOGY-
dc.contributor.googleauthorKim, Se Kye-
dc.contributor.googleauthorJung, Kyoung Hwa-
dc.contributor.googleauthorYoon, Sung Nyo-
dc.contributor.googleauthorKim, Yun Ki-
dc.contributor.googleauthorChai, Young Gyu-
dc.relation.code2016001720-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidygchai-
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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