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Details of the Collagen and Elastin Architecture in the Human Limbal Conjunctiva, Tenon’s Capsule and Sclera Revealed by Two-Photon Excited Fluorescence Microscopy

Title
Details of the Collagen and Elastin Architecture in the Human Limbal Conjunctiva, Tenon’s Capsule and Sclera Revealed by Two-Photon Excited Fluorescence Microscopy
Author
권지원
Keywords
collagen; conjunctiva; elastin; sclera; Tenon
Issue Date
2016-10
Publisher
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
Citation
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, v.57 ,NO.13, Page. 5602-5610
Abstract
PURPOSE. To investigate the architecture and distribution of collagen and elastin in human limbal conjunctiva, Tenon's capsule, and sclera. METHODS. The limbal conjunctiva, Tenon's capsule, and sclera of human donor corneal buttons were imaged with an inverted two-photon excited fluorescence microscope. No fixation process was necessary. The laser (Ti:sapphire) was tuned at 850 nm for two-photon excitation. Backscatter signals of second harmonic generation (SHG) and autofluorescence (AF) were collected through a 425/30-nm and a 525/45-nm emission filter, respectively. Multiple, consecutive, and overlapping (z-stack) images were acquired. Collagen signals were collected with SHG, whereas elastin signals were collected with AF. RESULTS. The size and density of collagen bundles varied widely depending on depth: increasing from conjunctiva to sclera. In superficial image planes, collagen bundles were <10 mu m in width, in a loose, disorganized arrangement. In deeper image planes (episclera and superficial sclera), collagen bundles were thicker (near 100 mu m in width) and densely packed. Comparatively, elastin fibers were thinner and sparse. The orientation of elastin fibers was independent of collagen fibers in superficial layers; but in deep sclera, elastin fibers wove through collagen interbundle gaps. At the limbus, both collagen and elastin fibers were relatively compact and were distributed perpendicular to the limbal annulus. CONCLUSIONS. Two-photon excited fluorescence microscopy has enabled us to understand in greater detail the collagen and elastin architecture of the human limbal conjunctiva, Tenon's capsule, and sclera.
URI
https://iovs.arvojournals.org/article.aspx?articleid=2577322https://repository.hanyang.ac.kr/handle/20.500.11754/100717
ISSN
0146-0404; 1552-5783
DOI
10.1167/iovs.16-19706
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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