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Oleic acid-embedded nanoliposome as a selective tumoricidal agent

Title
Oleic acid-embedded nanoliposome as a selective tumoricidal agent
Author
이은규
Keywords
LIMLET; HAMLET; Oleic acid; Liposome; Selective cytotoxicity; Tumoricide
Issue Date
2016-07
Publisher
ELSEVIER SCIENCE BV
Citation
COLLOIDS AND SURFACES B-BIOINTERFACES, v. 146, Page. 585-589
Abstract
HAMLET (Human Alpha-lactalbumin Made LEthal to Tumor cell), a molecular complex of human alpha-lactalbumin and oleic acid, is known to have selective cytotoxic activity against certain types of tumors. This cytotoxicity is known to stem from water-insoluble oleic acid. In this study, we manufactured an alternative complex using liposome as an oleic acid delivery vesicle. We named this nanolipoplex LIMLET (Liposome Made LEthal to Tumor cell). The LIMLET vesicle contained approximately 90,200 oleic acid molecules inserted into its lipophilic phospholipid bilayer and had a nominal mean diameter of 127 nm. Using a WST-1 assay, its cytotoxicity against two cancer cell lines, MDA-MB-231 (human breast cancer) and A549 (human lung cancer), were tested. The results were compared with that of a normal cell line, Vero (from monkey kidney). We found that (1) LIMLET showed distinctive cytotoxicity against A549 and MDA-MB-231 cells, whereas bare liposomes (containing no oleic acid) had no toxicity, even at high concentrations, and (2) LIMLET demonstrated selective, concentration-dependent toxicity against the cancer cells: the LD50 values of MDA-MB-231 and A549 cells were 1.3 and 2.2 nM LIMLET, respectively, whereas the LD50 of Vero was 5.7 nM. The strength of the tumoricidal effect appeared to stem from the number of oleic acid molecules present. Our result suggests that LIMLET, like HAMLET, is an interesting nanolipoplex that can potentially be developed into tumor treatments. (C) 2016 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S092777651630488Xhttps://repository.hanyang.ac.kr/handle/20.500.11754/100532
ISSN
0927-7765; 1873-4367
DOI
10.1016/j.colsurfb.2016.06.058
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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