Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신흥수 | - |
dc.date.accessioned | 2019-03-06T04:50:11Z | - |
dc.date.available | 2019-03-06T04:50:11Z | - |
dc.date.issued | 2016-10 | - |
dc.identifier.citation | JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, v. 12, NO 10, Page. 1916-1928 | en_US |
dc.identifier.issn | 1550-7033 | - |
dc.identifier.issn | 1550-7041 | - |
dc.identifier.uri | https://www.ingentaconnect.com/content/asp/jbn/2016/00000012/00000010/art00007 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/100516 | - |
dc.description.abstract | Construction of 3-dimensional (3-D) engineered tissue is increasingly being investigated for use in drug discovery and regenerative medicine. Here, we developed multi-layered 3-D cellular assembly by using magnetic nanoparticles (MNP) isolated from Magnetospirillum sp. AMB-1 magnetotactic bacteria. Magnetized human dermal fibroblasts (HDFBs) were prepared by treatment with the MNP, induced to form 3-D assembly under a magnetic field. Analyses including LIVE/DEAD assay, transmission electron microscopy revealed that the MNP were internalized via clathrin-mediated endocytosis without cytotoxicity. The magnetized HDFBs could build 3-D structure as a function of seeding density. When the highest seeding density (5 x 10(5) cells/mm(2)) was used, the thickness of assembly was 41.0 +/- 1.69 mu m, with approximately 9.3 +/- 1.6 cell layers being formed. Immunofluorescence staining confirmed homogeneous distribution of ECM and junction proteins throughout the 3-D assembly. Real-time PCR analysis showed decrease in expression levels of collagen types I and IV but increase in that of connexin 43 in the 3-D assembly compared with the 2-D culture. Finally, we demonstrated that the discernible layers can be formed hierarchically by serial assembly. In conclusion, our study showed that a multi-layered structure can be easily prepared using magnetically-assisted cellular assembly with highlighting cell-cell and cell-ECM communication. | en_US |
dc.description.sponsorship | This work was supported by a National Research Foundation of Korea (NRF) funded by the the Ministry of Science, ICT and Future Planning (NRF-2013R1A2A2A03067809) and Radiation Technology R&D program (NRF-2015M2A2A6021052). | en_US |
dc.language.iso | en | en_US |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | en_US |
dc.subject | Hierarchical Tissue Mimicry | en_US |
dc.subject | Multi-Layered Structure | en_US |
dc.subject | 3-D Cellular Assembly | en_US |
dc.subject | Magnetic Nanoparticles | en_US |
dc.subject | Cellular Internalization | en_US |
dc.title | Construction of 3-D Cellular Multi-Layers with Extracellular Matrix Assembly Using Magnetic Nanoparticles | en_US |
dc.type | Article | en_US |
dc.relation.no | 10 | - |
dc.relation.volume | 12 | - |
dc.identifier.doi | 10.1166/jbn.2016.2303 | - |
dc.relation.page | 1916-1928 | - |
dc.relation.journal | JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | - |
dc.contributor.googleauthor | Lee, Yu Bin | - |
dc.contributor.googleauthor | Lee, Joong-Yup | - |
dc.contributor.googleauthor | Ahmad, Taufiq | - |
dc.contributor.googleauthor | Bak, Seongwoo | - |
dc.contributor.googleauthor | Lee, Jinkyu | - |
dc.contributor.googleauthor | Kim, Hwan D | - |
dc.contributor.googleauthor | Park, Tai Hyun | - |
dc.contributor.googleauthor | Hwang, Nathaniel S. | - |
dc.contributor.googleauthor | Lim, Youn Mook | - |
dc.contributor.googleauthor | Shin, Heungsoo | - |
dc.relation.code | 2016000346 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | hshin | - |
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