TY - JOUR AU - 신흥수 DA - 2015/10 PY - 2015 UR - http://pubs.rsc.org/en/Content/ArticleLanding/2015/RA/C5RA01626F#!divAbstract UR - http://hdl.handle.net/20.500.11754/28416 AB - In this work, we present a biomimetic fibrous scaffold containing two biomolecules. A biocompatible poly(L-lactide-co-epsilon-caprolactone) mesh was fabricated by an electrospinning method, and then acrylic acid was grafted on the mesh to introduce a carboxyl group through gamma-ray irradiation. Subsequently, the epidermal growth factor (EGF) and gelatin were coupled to the mesh through the EDC reaction. The modified mesh presents a consistent fibre diameter (874.4 + 178.5 nm), with carboxyl groups (1.3 mM). EGF (171.7 ng mg(-1) mesh) and gelatin (67.2 +/- 30.5 mu g mg(-1) mesh) were successfully coupled on the mesh. The coupled EGF and gelatin promoted the cell viability 1.5- times higher than that from a non-modified mesh. In particular, the EGF on the meshes independently allowed hMSC to present a 3-times greater involucrin expression and enabled improved procollagen secretion, implying trans-differentiation of hMSC to keratinocyte-like cells. Therefore, the co-immobilization strategy of biomolecules using radiation technology may be an alternative tool for tissue engineering applications. PB - ROYAL SOC CHEMISTRY KW - TISSUE ENGINEERING APPLICATIONS KW - EXTRACELLULAR-MATRIX KW - ENDOTHELIAL-CELLS KW - ACRYLIC-ACID KW - SCAFFOLDS KW - PROLIFERATION KW - COLLAGEN KW - FABRICATION KW - DIFFERENTIATION KW - NANOFIBER TI - Modulation of human mesenchymal stem cell survival on electrospun mesh with co-immobilized epithelial growth factor and gelatin IS - 69 VL - 5 DO - 10.1039/c5ra01626f T2 - RSC ADVANCES ER -