TY - JOUR AU - 주재범 DA - 2015/06 PY - 2015 UR - http://www.sciencedirect.com/science/article/pii/S0304389415004379 UR - http://hdl.handle.net/20.500.11754/25802 AB - Francisella tularensis is a human zoonotic pathogen and the causative agent of tularemia, a severe infectious disease. Given the extreme infectivity of F. tularensis and its potential to be used as a biological warfare agent, a fast and sensitive detection method is highly desirable. Herein, we construct a novel detection platform composed of two units: (I) Magnetic beads conjugated with multiple capturing antibodies against F. tularensis for its simple and rapid separation and (2) Genetically-engineered apoferritin protein constructs conjugated with multiple quantum dots and a detection antibody against F. tularensis for the amplification of signal. We demonstrate a 10-fold increase in the sensitivity relative to traditional lateral flow devices that utilize enzyme-based detection methods. We ultimately envision the use of our novel nanoprobe detection platform in future applications that require the highly-sensitive on-site detection of high-risk pathogens. (C) 2015 Elsevier B.V. All rights reserved. PB - ELSEVIER SCIENCE BV KW - Nanoprobes KW - Francisella tularensis KW - Apoferritin KW - Immunoassay KW - Biosensing TI - A novel nanoprobe for the sensitive detection of Francisella tularensis VL - 298 DO - 10.1016/j.jhazmat.2015.05.041 T2 - JOURNAL OF HAZARDOUS MATERIALS ER -