최재훈
2018-03-12T05:46:06Z
2018-03-12T05:46:06Z
2013-06
Plos one, 2013, 8(6), P.e66970
1932-6203
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0066970
http://hdl.handle.net/20.500.11754/45389
Recently, it has been demonstrated that high cholesterol diet induced hypercholesterolemia and vascular lipid oxidation and accumulation in zebrafish larvae, suggesting that zebrafish is a new promising atherosclerosis model in addition to mouse models. However, up to date, there was no report regarding inflammatory cytokine expression during the lipid accumulation in zebrafish larva and adult fish. In this study, we first demonstrated the expression levels of IL-1 beta and TNF-alpha in high cholesterol diet (HCD)-fed zebrafish larvae, and found that although HCD induced vascular lipid accumulation, the cytokine expressions in the larvae were not changed by HCD. Furthermore, there was no significant difference in leukocyte accumulation in vessels between control and HCD fed group. But prolonged HCD induced IL-1 beta expression in spleen and liver compared to those of control zebrafish, and produced very early stage of fatty streak lesion in dorsal aorta of 19 week HCD-fed zebrafish. These results indicate that HCD induced hypercholesterolemia and atherosclerotic changes in zebrafish are very early stage, and suggest the necessity of the generation of mutant zebrafish having a disruption in a lipid metabolism-related gene leading to severe hypercholesterolemia and advanced atherosclerosis.
This work was funded by the Ministry of Education, Science and Technology, Korea (2011-0005843) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology, South Korea (2011-0013669). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
en
National Institutes of Health
Animals
Blood Vessels
drug effects
immunology
metabolism
Cholesterol, adverse effects
Diet
High-Fat
Female
Gene Expression Regulation
Hypercholesterolemia
etiology, genetics
Immunity
Innate
Interleukin-1beta
Larva, Leukocytes
Male
Mice
Time Factors
Tumor Necrosis Factor-alpha
Zebrafish
Zebrafish Proteins
High Cholesterol Diet Induces IL-1 beta Expression in Adult but Not Larval Zebrafish
Article
6
8
10.1371/journal.pone.0066970
1-8
PLOS ONE
Yoon, Yina
Yoon, Jihye
Jang, Man-Young
Na, Yirang
Ko, Youngho
Choi, Jae-Hoon
Seok, Seung Hyeok
2013007124
S
COLLEGE OF NATURAL SCIENCES[S]
DEPARTMENT OF LIFE SCIENCE
jchoi75