Ramakrishna Suresh
2017-11-09T05:44:10Z
2017-11-09T05:44:10Z
2016-01
STEM CELLS INTERNATIONAL, Article number 6705927, Page. 1-14
1687-966X
1687-9678
https://www.hindawi.com/journals/sci/2016/6705927/
http://hdl.handle.net/20.500.11754/30610
Ubiquitination of core stem cell transcription factors can directly affect stem cell maintenance and differentiation. Ubiquitination and deubiquitination must occur in a timely and well-coordinated manner to regulate the protein turnover of several stemness related proteins, resulting in optimal embryonic stem cell maintenance and differentiation. There are two switches: an E3 ubiquitin ligase enzyme that tags ubiquitin molecules to the target proteins for proteolysis and a second enzyme, the deubiquitinating enzyme (DUBs), that performs the opposite action, thereby preventing proteolysis. In order to maintain stemness and to allow for efficient differentiation, both ubiquitination and deubiquitination molecular switches must operate properly in a balanced manner. In this review, we have summarized the importance of the ubiquitination of core stem cell transcription factors, such as Oct3/4, c-Myc, Sox2, Klf4, Nanog, and LIN28, during cellular reprogramming. Furthermore, we emphasize the role of DUBs in regulating core stem cell transcriptional factors and their function in stem cell maintenance and differentiation. We also discuss the possibility of using DUBs, along with core transcription factors, to efficiently generate induced pluripotent stem cells. Our review provides a relatively new understanding regarding the importance of ubiquitination/deubiquitination of stem cell transcription factors for efficient cellular reprogramming.
The authors sincerely thank Suri's Lab members for helpful discussion. This study was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean Government (MSIP) (no. 2012M3A9B4028738).
en
HINDAWI PUBLISHING CORP
PLURIPOTENT STEM-CELLS
HUMAN SOMATIC-CELLS
C-MYC PROTEOLYSIS
PROTEIN-DEGRADATION
DEFINED FACTORS
IPS CELLS
TRANSCRIPTION FACTORS
HUMAN FIBROBLASTS
SLEEPING-BEAUTY
SELF-RENEWAL
The Importance of Ubiquitination and Deubiquitination in Cellular Reprogramming
Article
10.1155/2016/6705927
1-14
STEM CELLS INTERNATIONAL
Suresh, Bharathi
Lee, Junwon
Kim, Kye-Seong
Ramakrishna, Suresh
2016011215
S
Graduate School of Biomedical Science & Engineering[S]
suri28