이영호
2017-10-24T01:42:52Z
2017-10-24T01:42:52Z
2015-12
NEURAL REGENERATION RESEARCH, v. 10, NO 12, Page. 2018-2024
1673-5374
1876-7958
http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=12;spage=2018;epage=2024;aulast=Koh
http://hdl.handle.net/20.500.11754/30214
To investigate a possible therapeutic mechanism of cell therapy in the field of cerebral palsy using granulocyte-colony stimulating factor (G-CSF)-mobilized peripheral blood mononuclear cells (mPBMCs), we compared the expression of inflammatory cytokines and neurotrophic factors in PBMCs and mPBMCs from children with cerebral palsy to those from healthy adult donors and to cord blood mononuclear cells donated from healthy newborns. No significant differences in expression of neurotrophic factors were found between PBMCs and mPBMCs. However, in cerebral palsy children, the expression of interleukin-6 was significantly increased in mPBMCs as compared to PBMCs, and the expression of interleukin-3 was significantly decreased in mPBMCs as compared to PBMCs. In healthy adults, the expression levels of both interleukin-1 beta and interleukin-6 were significantly increased in mPBMCs as compared to PBMCs. The expression of brain-derived neurotrophic factors in mPBMC from cerebral palsy children was significantly higher than that in the cord blood or mPBMCs from healthy adults. The expression of G-CSF in mPBMCs from cerebral palsy children was comparable to that in the cord blood but significantly higher than that in mPBMCs from healthy adults. Lower expression of pro-inflammatory cytokines (interleukin-1 beta, interleukin-3, and -6) and higher expression of anti-inflammatory cytokines (interleukin-8 and interleukin-9) were observed from the cord blood and mPBMCs from cerebral palsy children rather than from healthy adults. These findings indicate that mPBMCs from cerebral palsy and cord blood mononuclear cells from healthy newborns have the potential to become seed cells for treatment of cerebral palsy.
This work was supported by the Research Fund of Hanyang University (HY-2012).
en
SHENYANG EDITORIAL DEPT NEURAL REGENERATION RES
neurotrophic factors
inflammatory cytokines
cord blood
G-CSF mobilized peripheral blood
mononuclear cell
cerebral palsy
children
neural regeneration
Mononuclear cells from the cord blood and granulocyte-colony stimulating factor-mobilized peripheral blood: is there a potential for treatment of cerebral palsy?
Article
12
10
10.4103/1673-5374.172321
2018-2024
NEURAL REGENERATION RESEARCH
Koh, Hani
Hwang, Kyoujung
Lim, Hae-Young
Kim, Yong-Joo
Lee, Young-Ho
2015006272
S
COLLEGE OF MEDICINE[S]
DEPARTMENT OF MEDICINE
cord
0000-0003-1498-2773