진언선
2016-10-13T06:20:34Z
2016-10-13T06:20:34Z
2015-04
ENVIRONMENTAL SCIENCE & TECHNOLOGY, v. 49, NO 7, Page. 4466-4472
0013-936X
1520-5851
http://pubs.acs.org/doi/abs/10.1021/es505143f
http://hdl.handle.net/20.500.11754/23776
We developed a process for one-pot CO2 conversion and utilization based on simple conversion of CO2 to bicarbonate at ambient temperature with no energy input, by using the cross-linking-based composites of carboxylated polyaniline nanofibers (cPANFs) and carbonic anhydrase. Carbonic anhydrase was immobilized on cPANFs via the approach of magnetically separable enzyme precipitate coatings (Mag-EPC), which consists of covalent enzyme attachment, enzyme precipitation, and cross-linking with amine-functionalized magnetic nanoparticles. Mag-EPC showed a half-life of 236 days under shaking, even resistance to 70% ethanol sterilization, and recyclability via facile magnetic separation. For one-pot CO2 conversion and utilization, Mag-EPC was used to accelerate the growth of microalga by supplying bicarbonate from CO2, representing 1.8-fold increase of cell concentration when compared to the control sample. After two repeated uses via simple magnetic separation, the cell concentration with Mag-EPC was maintained as high as the first cycle. This one-pot CO2 conversion and utilization is an alternative as well as complementary process to adsorption-based CO2 capture and storage as an environmentally friendly approach, demanding no energy input based on the effective action of the stabilized enzyme system.
This work was supported by grants from the International Collaborative R&D Program (No. 20118510020020) and Energy Efficiency & Resources Core Technology Program (No. 20142020200980) of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), funded by the Korea government Ministry of Trade, Industry & Energy. This work was also supported by the Korea CCS R&D Center (KCRC) (NRF-2014M1A8A1049273), by the Advanced Biomass R&D Centre (ABC) of Global Frontier Project (ABC-M3A6A2079376), by the Nano-Material Technology Development Program (2014M3A7B4052193), and by the Global Research Laboratory Program (2014K1A1A2043032), all of which are funded by the Korea government Ministry of Science, ICT & Future Planning.
en
AMER CHEMICAL SOC
ALGA DUNALIELLA-SALINA
CO2 CAPTURE
ANHYDRASE;
MICROALGAE
One-Pot Enzymatic Conversion of Carbon Dioxide and Utilization for Improved Microbial Growth
Article
7
49
10.1021/es505143f
4466-4472
ENVIRONMENTAL SCIENCE & TECHNOLOGY
Hong, Sung-Gil
Jeon, Hancheol
Kim, Han Sol
Jun, Seung-Hyun
Jin, EonSeon
Kim, Jungbae
2015000314
S
COLLEGE OF NATURAL SCIENCES[S]
DEPARTMENT OF LIFE SCIENCE
esjin