배상수
2019-04-17T07:47:44Z
2019-04-17T07:47:44Z
2016-12
ANALYTICAL CHEMISTRY, v. 88, NO 23, Page. 11288-11292
0003-2700
1520-6882
https://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b03320
https://repository.hanyang.ac.kr/handle/20.500.11754/102286
Surface enhanced Raman scattering (SERS) is highly useful for sensitive analytical sensing; however, its practical availability for detecting a point mutation associated with disease in clinical sample was rarely proved. Herein, we present a toehold-mediated, DNA displacement-based, SERS sensor for detecting point mutations in the BIGH3 gene associated with the most common corneal dystrophies (CDs) in a clinical setting. To diagnose Avellino corneal dystrophy (ACD), selectivity was ensured by exploring optimal DNA displacement conditions such as length of toehold and hybridization temperature. A SERS-efficient Ag@Au bimetallic nanodendrite was employed to ensure sensitivity. Optimization for a clinical setting showed that discrimination was maximized when toehold length was 6-mer (T6), and hybridization temperature was 36 degrees C. On the basis of tests that used clinical homozygous and heterozygous CD samples, a single-base mismatched DNA sequence was identifiable within 30 min with a limit of detection (LOD) of 400 fM. From the results, we conclude that our toehold-mediated, DNA displacement-based, SERS sensor allows a rapid and sensitive detection of a BIGH3 gene point mutation associated with Avellino corneal dystrophy, indicating the practical ability of the method to diagnose genetic diseases caused by point mutations.
This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (NRF-2014S1A2A2027641) and by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2015R1A2A2A01006445). Further support by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI16C1067) is appreciated.
en
AMER CHEMICAL SOC
DNA
GENE
CHIP
NANOPARTICLES
HYBRIDIZATION
EXACERBATION
APTAMER
PROBES
LASIK
Sensitive Surface Enhanced Raman Scattering-Based Detection of a BIGH3 Point Mutation Associated with Avellino Corneal Dystrophy
Article
23
88
10.1021/acs.analchem.6b03320
11288-11292
ANALYTICAL CHEMISTRY
Kim, Saetbyeol
Jeong, Su-Nam
Bae, Sangsu
Chung, Hoeil
Yoo, So Young
2016002866
S
COLLEGE OF NATURAL SCIENCES[S]
DEPARTMENT OF CHEMISTRY
sangsubae
E-5324-2017
http://orcid.org/0000-0003-3615-8566