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Differences of Molecular Mechanisms between Ruptured and Non-Ruptured Carotid Atherosclerotic Plaques, and the Significance of ABCA1.

Title
Differences of Molecular Mechanisms between Ruptured and Non-Ruptured Carotid Atherosclerotic Plaques, and the Significance of ABCA1.
Other Titles
파열과 비파열 경동맥 죽상경화판의 분자기전 차이 및 ABCA1의 중요성
Author
Eun-Hye Lee
Alternative Author(s)
이은혜
Advisor(s)
고성호
Issue Date
2019-02
Publisher
한양대학교
Degree
Doctor
Abstract
Carotid plaques are a strong risk factor for ischemic stroke, and in terms of plaque rupture, the risk goes even higher. In spite of the significance of carotid plaques, few have studied the differences in molecular mechanisms between ruptured and non-ruptured carotid plaques. Previous studies have focused on identifying the crucial target molecules in ruptured plaques, since early diagnosis and therapy for carotid plaque rupture is important to prevent ischemic stroke. However, critical factors for symptomatic ruptured plaques are yet to be identified. In this study, 79 carotid plaques were collected and microscopically determined as ruptured or non-ruptured plaques. For examination of RNA and protein dynamics in plaques, quantitative polymerase chain reaction (qPCR) array, proteomics, and immunohistochemistry (IHC) were conducted to compare their molecular expression. According to the results, they revealed that levels of several factors, including ATP-binding cassette subfamily A member 1 (ABCA1), were significantly higher in ruptured plaques than in non-ruptured ones. To identify the utility of ABCA1 as a clinical biomarker in carotid rupture, enzyme-linked immunosorbent assay (ELISA) was performed using patients’ sera. The expression of ABCA1 was consistently demonstrated in the serum of patients with symptomatic ruptured plaques, while those with non-ruptured plaques were all negative for ABCA1. Taken together, our results suggest a plausible mechanism involving carotid plaque rupture, and point out ABCA1 as a biomarker for symptomatic carotid plaque rupture.
URI
http://dcollection.hanyang.ac.kr/common/orgView/000000108797http://repository.hanyang.ac.kr/handle/20.500.11754/99946
Appears in Collections:
GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S](의생명공학전문대학원) > TRANSLATIONAL MEDICAL SCIENCE(임상의과학과) > Theses (Ph.D.)
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