Phthalate-induced cytotoxicity and functional impairment in bEnd.3 cells.

Title
Phthalate-induced cytotoxicity and functional impairment in bEnd.3 cells.
Author
Haram Kim
Alternative Author(s)
김하람
Advisor(s)
배옥남
Issue Date
2019-02
Publisher
한양대학교
Degree
Master
Abstract
As the exposure level to phthalates, which are widely used plasticizers, has increased, the concerns for their adverse health effects have been expanded. Although the most sensitive phthalate toxicity is known to be male reproductive/developmental toxicity, the association between phthalates and other diseases such as stroke has been also reported. The effects of phthalates on brain endothelial cells, which are important components of blood-brain barrier (BBB) are largely unknown. Here we investigated if phthalates affect brain endothelial cell viability permeability, and if phthalates increase the susceptibility of brain endothelial damage induced by in vitro ischemic condition of oxygen-glucose deprivation (OGD). In brain endothelial cell line bEnd.3, three representative types of phthalates, di-ethylhexyl phthalate (DEHP), dibutyl phthalate (DBP) and diisobutyl phthalate (DIBP), increased cytotoxicity and the susceptibility of OGD-induced damage, with different potency. The functional integrity of BBB, as determined by permeability assay in trans-well system, has been disrupted by phthalates. Potential mechanisms including degradation of tight junction proteins and impairment of mitochondrial membrane potential were examined. Taken together, we demonstrated that phthalates induce brain endothelial damage and disturbance in permeability, suggesting that these effects can contribute to phthalate-induced cardiovascular damage or ischemic stroke. Further studies warrant the identification of underlying mechanisms and the in vivo effects of phthalates on BBB function.
URI
http://dcollection.hanyang.ac.kr/common/orgView/000000109001http://repository.hanyang.ac.kr/handle/20.500.11754/99417
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > PHARMACY(약학과) > Theses (Master)
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