Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김철영 | - |
dc.date.accessioned | 2019-01-22T01:37:24Z | - |
dc.date.available | 2019-01-22T01:37:24Z | - |
dc.date.issued | 2018-09 | - |
dc.identifier.citation | MOLECULES, v. 23, No. 9, Article no. 2274 | en_US |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | https://www.mdpi.com/1420-3049/23/9/2274/htm | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/81397 | - |
dc.description.abstract | Activity-guided separation of antioxidant response element (ARE)-inducing constituents from the rhizomes of Atractylodis Rhizoma Alba was performed by the combination of centrifugal partition chromatography (CPC) and an ARE luciferase reporter assay. From 3 g of the active n-hexane fraction, one polyacetylene, (6E, 12E)-tetradeca-6,12-dien-8,10-diyne-1,3-diyl diacetate (47.3 mg), and two sesquiterpenes, atractylenolide I (40.9 mg), and selina-4(14), 7(11)-dien-8-one (6.0 mg) were successfully isolated by CPC with n-hexane-ethyl acetate-methanol-water (8: 2: 8: 2, v/v). The chemical structures of the isolated compounds were determined by H-1- and C-13-NMR and ESI-MS. Among the isolated compounds, (6E, 12E)-tetradeca-6,12-diene-8,10-diyne-1,3-diol diacetate and selina-4(14), 7(11)-dien-8-one increased ARE activity 32.9-fold and 16.6-fold, respectively, without significant cytotoxicity, when 5 mu M sulforaphane enhanced ARE activity 27.1-fold. However, atractylenolide I did not increase ARE activity at 100 mu M, and showed cytotoxicity at concentrations over 10 mu M. | en_US |
dc.description.sponsorship | This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2016R1A2B4014598). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | MDPI AG | en_US |
dc.subject | Atractylodis Rhizoma Alba | en_US |
dc.subject | centrifugal partition chromatography | en_US |
dc.subject | antioxidant response element | en_US |
dc.subject | polyacetylene | en_US |
dc.subject | sesquiterpenes | en_US |
dc.title | Application of Centrifugal Partition Chromatography for Bioactivity-Guided Purification of Antioxidant-Response-Element-Inducing Constituents from Atractylodis Rhizoma Alba | en_US |
dc.type | Article | en_US |
dc.relation.no | 9 | - |
dc.relation.volume | 23 | - |
dc.identifier.doi | 10.3390/molecules23092274 | - |
dc.relation.page | 1-10 | - |
dc.relation.journal | MOLECULES | - |
dc.contributor.googleauthor | Kim, Myeong Il | - |
dc.contributor.googleauthor | Kim, Ji Hoon | - |
dc.contributor.googleauthor | Syed, Ahmed Shah | - |
dc.contributor.googleauthor | Kim, Young-Mi | - |
dc.contributor.googleauthor | Choe, Kevin Kyungsik | - |
dc.contributor.googleauthor | Kim, Chul Young | - |
dc.relation.code | 2018007216 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | chulykim | - |
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