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dc.contributor.author김동욱-
dc.date.accessioned2018-12-14T04:31:27Z-
dc.date.available2018-12-14T04:31:27Z-
dc.date.issued2008-12-
dc.identifier.citationMOLECULAR IMMUNOLOGY, v. 45, No. 11, Page. 3295-3301en_US
dc.identifier.issn0161-5890-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0161589008000771-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/80861-
dc.description.abstractShigella spp., Gram-negative pathogenic bacteria, deliver various effector molecules into the host cell cytoplasm through their type III secretion system to facilitate their invasive process and control the host innate immune responses. Although the function of these effectors is well characterized in epithelial cells during Shigella infection, it has not been elucidated in the dendritic cell (DC), a major antigen presenting cell playing an important role in the initiation of immune responses. In this study, we showed that an invasive Shigella strain (M90T), but not its non-invasive counterpart strain (BS176) induced apoptotic cell death in the human monocyte-derived DCs. Confocal microscopy using a lysosome-associated membrane protein 2 specific antibody demonstrated that the M90T escaped from phagosomes 2h post-DC invasion while BS176 remained in the phagosome. Furthermore, Shigella expressed outer Shigella protein F(OspF), one of the effector proteins that are released through type III secretion system during the invasion, at non-secretion state and further up-regulated OspF expression in the cytoplasm of DC during the invasion. Interestingly, in the host cell, OspF could directly bind to the extracellular signal-regulated kinase (Erk) 1/2 and dephosphorylate phospho-Erk. These results suggest that induction of OspF is enhanced during Shigella invasion of DCs and decreases the phosphorylation level of Erk1/2, which could be at least partially involved in the apoptotic death of DC, eventually resulting in the down-regulation of the host immune response. (C) 2008 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipThis work was supported by a grant from the Biogreen 21 program (2005 04 01034696), Rural Development Administration and GRCMVP for Technology Development Program of Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea.en_US
dc.language.isoen_USen_US
dc.publisherPERGAMON-ELSEVIER SCIENCE LTDen_US
dc.subjectdendritic cellsen_US
dc.subjectShigellaen_US
dc.subjectextracellular signal-regulated kinasesen_US
dc.subjectOspFen_US
dc.titleOspF directly attenuates the activity of extracellular signal-regulated kinase during invasion by Shigella flexneri in human dendritic cellsen_US
dc.typeArticleen_US
dc.relation.volume45-
dc.identifier.doi10.1016/j.molimm.2008.02.013-
dc.relation.page3295-3301-
dc.relation.journalMOLECULAR IMMUNOLOGY-
dc.contributor.googleauthorKim, Dong Wook-
dc.contributor.googleauthorChu, Hyuk-
dc.contributor.googleauthorJoo, Dong Hyun-
dc.contributor.googleauthorJang, Mi Seon-
dc.contributor.googleauthorChoi, Jin Huk-
dc.contributor.googleauthorPark, Sung-Moo-
dc.contributor.googleauthorChoi, Yun-Jae-
dc.contributor.googleauthorHan, Seung Hyun-
dc.contributor.googleauthorYun, Cheol-Heui-
dc.relation.code2008206821-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF PHARMACY[E]-
dc.sector.departmentDEPARTMENT OF PHARMACY-
dc.identifier.piddongwook-
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COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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