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dc.contributor.author유혜현-
dc.date.accessioned2018-12-14T01:37:41Z-
dc.date.available2018-12-14T01:37:41Z-
dc.date.issued2008-12-
dc.identifier.citationARCHIVES OF PHARMACAL RESEARCH, v. 31, No. 12, Page. 1644-1651en_US
dc.identifier.issn0253-6269-
dc.identifier.urihttps://link.springer.com/article/10.1007/s12272-001-2163-5-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/80860-
dc.description.abstractA liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-ESI-MS/MS) method was developed and validated for the simultaneous detection and quantification of seven amphetamine derivatives (amphetamine (AP), methamphetamine (MA), 3,4-methylenedioxy-N-amphetamine (MDA), 3,4-methylenedioxy-N-methamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), N,N-dimethylamphetamine (DMA), and N,N-dimethylamphetamine-N-oxide (DMANO)) in human urine. Seven deuterium-labeled compounds were prepared for use as internal standards to quantify the analytes. One milliliter of urine was combined with 1 mL of 0.2 M sodium carbonate buffer solution (pH 9.0) before solid phase extraction (SPE). An Oasis HLB SPE column followed by chromatographic separation on a Capcell Pak C18 MG-II column (150 x 2.0 mm I.D., 5 mu m) and electrospray mass spectrometry with multiple reaction monitoring were used for selective and sensitive detection. The use of ammonium formate (5 mM, pH adjusted to 4.0 with formic acid, Solvent A) and acetonitrile (Solvent B) as the mobile phase at a flow rate of 230 mu L/min was found to be the most effective for the separation. The linear ranges were 5.0-1000 ng/mL for AP, MDA, MDMA, MDEA, DMA, and DMANO and 10.0-1000 ng/mL for MA, with good correlation coefficients (r(2) > 0.996). The intra-day, inter-day, and interperson precisions were within 14.6%, 12.1% and 15.5%, respectively. The intra-day, inter-day, and interperson accuracies were between -11.6 and 9.0%, -7.9 and 2.3%, and -13.2 and 4.3%, respectively. The limits of detection (LODs) for each analytical compound were lower than 1.95 ng/mL. The recovery ranged from 72.3 to 103.3%. The applicability of the developed method was examined by analyzing several urine samples from confirmed drug abusers.en_US
dc.description.sponsorshipThis study was supported in part by the grant (M10640010000-06N4001-00100) from National R& D program of Ministry of Education, Science and Technology (MEST) and Korea Science and Engineering Foundation (KOSEF).en_US
dc.language.isoen_USen_US
dc.publisherPHARMACEUTICAL SOCIETY KOREAen_US
dc.subjectMethamphetamineen_US
dc.subjectDimethylamphetamineen_US
dc.subjectMDMAen_US
dc.subjectMDEAen_US
dc.subjectLC-MS/MSen_US
dc.subjectUrineen_US
dc.titleSimultaneous determination of methamphetamine, 3,4-methylenedioxy-N-methylamphetamine, 3,4-methylenedioxy-N-ethylamphetamine, N,N-dimethylamphetamine, and their metabolites in urine by liquid chromatography-electrospray ionization-tandem mass spectrometryen_US
dc.typeArticleen_US
dc.relation.volume31-
dc.identifier.doi10.1007/s12272-001-2163-5-
dc.relation.page1644-1651-
dc.relation.journalARCHIVES OF PHARMACAL RESEARCH-
dc.contributor.googleauthorKim, Jin Young-
dc.contributor.googleauthorCheong, Jae Chul-
dc.contributor.googleauthorKo, Beom Jun-
dc.contributor.googleauthorLee, Sang Kyu-
dc.contributor.googleauthorYoo, Hye Hyun-
dc.contributor.googleauthorJin, Changbae-
dc.contributor.googleauthorIn, Moon Kyo-
dc.relation.code2008200969-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF PHARMACY[E]-
dc.sector.departmentDEPARTMENT OF PHARMACY-
dc.identifier.pidyoohh-
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COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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