Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 유혜현 | - |
dc.date.accessioned | 2018-12-14T01:34:02Z | - |
dc.date.available | 2018-12-14T01:34:02Z | - |
dc.date.issued | 2008-12 | - |
dc.identifier.citation | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v. 48, No. 5, Page. 1425-1429 | en_US |
dc.identifier.issn | 0731-7085 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0731708508004937 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/80859 | - |
dc.description.abstract | A simple, rapid, and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was applied to pharmacokinetic study of a neuroactive oleanolic-glycoside saponin, hederacolchi side E from SK-P-B70M, a standardized extract of Pulsatilla koreana in rat. Rat plasma samples were pretreated by protein precipitation with acetonitrile, eluted from C-18 column, and analyzed using electrospray ionization (ESI)-MS/MS in negative ion mode. Digoxin was used as an internal standard. The standard curves were linear (r > 0.997) over the concentration ranges of 2-500 ng/mL. The intra- and inter-day precisions were measured to be below 9% and accuracy between 90 and 111% for all quality control samples at 2, 20, 100, and 500 ng/mL (n = 5). The lower limits of quantification (LLOQ) for hederacolchiside E was 2 ng/mL and the limit of detection (LOD) 0.5 ng/mL using 20 mu L of plasma sample. Subsequently, hederacolchiside E was determined in rat plasma samples after oral administration of SK-PC-B70M. The mean maximum plasma concentrations of hederacolchiside E were 0.07, 0.13, and 0.36 mu g/mL and the mean areas under the plasma concentration versus time curve 0.56, 1.27, and 6.46 mu g h/mL at doses of 100, 200, and 400mg/kg, respectively, which indicated non-linear pharmacokinetic pattern. In conclusion, this method was successfully applied to the pharmacokinetic study of hederacolchiside E after an oral administration of SK-PC-B70M to rats. (C) 2008 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by a grant from Korea health R&D project from Ministry of Health and Welfare, Republic of Korea (A050580). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | en_US |
dc.subject | LC-MS/MS | en_US |
dc.subject | Hederacolchiside E | en_US |
dc.subject | Pulsatilla koreano | en_US |
dc.subject | SK-PC-B70M | en_US |
dc.subject | Pharmacokinetic study | en_US |
dc.subject | MASS-SPECTROMETRY | en_US |
dc.title | LC-MS/MS method for determination of hederacolchiside E, a neuroactive saponin from Pulsatilla koreana extract in rat plasma for pharmacokinetic study | en_US |
dc.type | Article | en_US |
dc.relation.volume | 48 | - |
dc.identifier.doi | 10.1016/j.jpba.2008.09.012 | - |
dc.relation.page | 1425-1429 | - |
dc.relation.journal | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | - |
dc.contributor.googleauthor | Yoo, Hye Hyun | - |
dc.contributor.googleauthor | Lee, Sang Kyu | - |
dc.contributor.googleauthor | Lim, Sun Young | - |
dc.contributor.googleauthor | Kim, Yoon | - |
dc.contributor.googleauthor | Kang, Min Jung | - |
dc.contributor.googleauthor | Kim, Eun Jeong | - |
dc.contributor.googleauthor | Park, Yang Hae | - |
dc.contributor.googleauthor | Im, Guang-Jin | - |
dc.contributor.googleauthor | Lee, Bong Yong | - |
dc.contributor.googleauthor | Kim, Dong-Hyun | - |
dc.relation.code | 2008205623 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | yoohh | - |
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