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Identification of Glutathione Conjugates of 1-Bromopentane and its Hepatotoxicity in Female BALB/c Mice

Title
Identification of Glutathione Conjugates of 1-Bromopentane and its Hepatotoxicity in Female BALB/c Mice
Author
유혜현
Keywords
1-Bromopentane; Glutathione; Conjugate structure; Hepatotoxicity; In vivo; Mice
Issue Date
2008-10
Publisher
PHARMACEUTICAL SOCIETY KOREA
Citation
ARCHIVES OF PHARMACAL RESEARCH, v. 31, No. 10, Page. 1317-1323
Abstract
Halogenated organic compounds, such as 1-bromopentane (1-BPT), are used as cleaning agents, synthesis agents, or extraction solvents in the workplace. In the present study, glutathione (GSH) conjugation and hepatotoxicity induced by 1-BPT were investigated in female BALB/c mice. S-Bromopentyl GSH, S-bromopentyl cysteine, and mono-hydroxypentyl mercapturic acid were identified in liver by liquid chromatography-electrospray ionization tandem mass spectrometry. Oral treatment of mice with 1-BPT at 1500 mg/kg produced maximum GSH conjugates at 6 h after treatment. For hepatotoxicity tests, the animals were treated orally with 1-BPT at 375, 750, or 1500 mg/kg in corn oil once for a dose response study or at 1500 mg/kg for 6, 12, 24, or 48 h for a time course study. 1-BPT dose-dependently increased serum activity of ALT and AST and decreased hepatic GSH levels, peaking at 6 and 12 h after treatment. 1-BPT (750 and 1500 mg/kg) also significantly increased the hepatic content of malondialdehyde. Thus, 1-BPT could cause hepatotoxicity and depletion of GSH content by forming GSH conjugates, presenting a toxicity mechanism and potential biomarkers for low molecular weight haloalkanes.
URI
https://link.springer.com/article/10.1007/s12272-001-2112-3http://repository.hanyang.ac.kr/handle/20.500.11754/80709
ISSN
0253-6269
DOI
10.1007/s12272-001-2112-3
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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