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Ulinastatin up-regulates glutamate transporters in glial cells

Title
Ulinastatin up-regulates glutamate transporters in glial cells
Author
이희종
Keywords
Glutamate uptake; glutamate transporters; ulinastatin
Issue Date
2016-09
Publisher
E-CENTURY PUBLISHING CORP
Citation
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, v. 9, NO. 2, Page. 2367-2374
Abstract
General anesthesia impairs glutamate homeostasis at synapses by decreasing the production of glutamate transporters. Glutamate transporters in astrocytes are important to preserve cognitive function and memory in hippocampus. In this study we investigated whether ulinastatin increases the number of glutamate transporters in glial cells. We used human astrocytes to demonstrate glutamate uptake in glial cells. The cells were divided into control and ulinastatin-treated groups. After incubation for 3 hours in serum free media, ulinastatin was administered to ulinastatin-treated group, then media was changed to HBSS buffer with 0.1 mM of glutamate. And we determined glutamate absorption by astrocytes by measuring glutamate levels in the media and astrocyte lysates at a time. We also measured levels of the glutamate transporters. In the ulinastatin-treated group, the total amount of glutamate was decreased, and the amount in astrocytes was significantly increased, compared to the control group. Expression of glutamate transporter 1 and glutamate aspartate transporter was significantly increased in the ulinastatin group. In this study, administration of ulinastatin resulted in increased glutamate uptake by astrocytes. We also demonstrated glutamate transporters. Thus, we propose that ulinastatin may improve short memory impairment and cognitive disorder after anesthesia by increasing the number of glutamate transporters.
URI
https://www.semanticscholar.org/paper/Ulinastatin-upregulates-glutamate-transporters-in-Min-Kim/97227d5c2706fe9a785a4348fa3d0ba6a474afaehttps://repository.hanyang.ac.kr/handle/20.500.11754/80610
ISSN
1940-5901
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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