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dc.contributor.author김진웅-
dc.date.accessioned2018-11-14T01:39:48Z-
dc.date.available2018-11-14T01:39:48Z-
dc.date.issued2016-09-
dc.identifier.citationLAB ON A CHIP, v. 16, NO. 17, Page. 3330-3339en_US
dc.identifier.issn1473-0197-
dc.identifier.issn1473-0189-
dc.identifier.urihttps://pubs.rsc.org/en/Content/ArticleLanding/2016/LC/C6LC00169F#!divAbstract-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/80389-
dc.description.abstractThe mechanical phenotype or 'mechanotype' of cells is emerging as a potential biomarker for cell types ranging from pluripotent stem cells to cancer cells. Using a microfluidic device, cell mechanotype can be rapidly analyzed by measuring the time required for cells to deform as they flow through constricted channels. While cells typically exhibit deformation timescales, or transit times, on the order of milliseconds to tens of seconds, transit times can span several orders of magnitude and vary from day to day within a population of single cells; this makes it challenging to characterize different cell samples based on transit time data. Here we investigate how variability in transit time measurements depends on both experimental factors and heterogeneity in physical properties across a population of single cells. We find that simultaneous transit events that occur across neighboring constrictions can alter transit time, but only significantly when more than 65% of channels in the parallel array are occluded. Variability in transit time measurements is also affected by the age of the device following plasma treatment, which could be attributed to changes in channel surface properties. We additionally investigate the role of variability in cell physical properties. Transit time depends on cell size; by binning transit time data for cells of similar diameters, we reduce measurement variability by 20%. To gain further insight into the effects of cell-to-cell differences in physical properties, we fabricate a panel of gel particles and oil droplets with tunable mechanical properties. We demonstrate that particles with homogeneous composition exhibit a marked reduction in transit time variability, suggesting that the width of transit time distributions reflects the degree of heterogeneity in subcellular structure and mechanical properties within a cell population. Our results also provide fundamental insight into the physical underpinnings of transit measurements: transit time depends strongly on particle elastic modulus, and weakly on viscosity and surface tension. Based on our findings, we present a comprehensive methodology for designing, analyzing, and reducing variability in transit time measurements; this should facilitate broader implementation of transit experiments for rapid mechanical phenotyping in basic research and clinical settings.en_US
dc.description.sponsorshipWe are grateful to the National Science Foundation (CAREER DBI-1254185) and the Farber Family Foundation for financial support. We also thank the Integrated Systems Nanofabrication Cleanroom at the California NanoSystems Institute and its staff for providing fabrication facilities. Thanks to Don and Ada Olins for the gift of the HL-60 cells and Angelyn Nguyen for critical reading of this manuscript and the pancreatic ductal epithelial (HPDE) cells.en_US
dc.language.isoenen_US
dc.publisherROYAL SOC CHEMISTRYen_US
dc.subjectPASSIVE MECHANICAL-BEHAVIORen_US
dc.subjectCANCER-CELLSen_US
dc.subjectVISCOELASTIC PROPERTIESen_US
dc.subjectHUMAN NEUTROPHILSen_US
dc.subjectFORCE MICROSCOPYen_US
dc.subjectMAMMALIAN-CELLSen_US
dc.subjectDEFORMABILITYen_US
dc.subjectNUCLEUSen_US
dc.subjectVISCOSITYen_US
dc.subjectCYTOMETRYen_US
dc.titleThe physical origins of transit time measurements for rapid, single cell mechanotypingen_US
dc.typeArticleen_US
dc.relation.no17-
dc.relation.volume16-
dc.identifier.doi10.1039/c6lc00169f-
dc.relation.page3330-3339-
dc.relation.journalLAB ON A CHIP-
dc.contributor.googleauthorNyberg, Kendra D.-
dc.contributor.googleauthorScott, Michael B.-
dc.contributor.googleauthorBruce, Samuel L.-
dc.contributor.googleauthorGopinath, Ajay B.-
dc.contributor.googleauthorBikos, Dimitri-
dc.contributor.googleauthorMason, Thomas G.-
dc.contributor.googleauthorKim, Jin Woong-
dc.contributor.googleauthorChoi, Hong Sung-
dc.contributor.googleauthorRowat, Amy C.-
dc.relation.code2016000617-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidkjwoong-
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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