Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 정봉근 | - |
dc.date.accessioned | 2018-10-30T02:18:44Z | - |
dc.date.available | 2018-10-30T02:18:44Z | - |
dc.date.issued | 2008-02 | - |
dc.identifier.citation | BIOMATERIALS, v. 29, No. 6, Page. 752-763 | en_US |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0142961207008320 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/76862 | - |
dc.description.abstract | Directed embryonic stem (ES) cell differentiation is a potentially powerful approach for generating a renewable source of cells for regenerative medicine. Typical in vitro ES cell differentiation protocols involve the formation of ES cell aggregate intermediates called embryoid bodies (EBs). Recently, we demonstrated the use of poly(ethylene glycol) (PEG) microwells as templates for directing the formation of these aggregates, offering control over parameters such as size, shape, and homogeneity. Despite these promising results, the previously developed technology was limited as it was difficult to reproducibly obtain cultures of homogeneous EBs with high efficiency and retrievability. In this study, we improve the platform by optimizing a number of features: material composition of the microwells, cell seeding procedures, and aggregate retrieval methods. Adopting these modifications, we demonstrate an improved degree of homogeneity of the resulting aggregate populations and establish a robust protocol for eliciting high EB formation efficiencies. The optimized microwell array system is a potentially versatile tool for ES cell differentiation studies and high-throughput stem cell experimentation. | en_US |
dc.description.sponsorship | We would like to thank Judy Yeh for helpful assistance and discussion. This research has been supported by the NIH, the Coulter Foundation, the Draper Laboratory, and the Institute for Soldier Nanotechnology (DAAD- 19-02-D-002). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER SCI LTD | en_US |
dc.subject | microwells | en_US |
dc.subject | poly(ethylene glycol) (PEG) | en_US |
dc.subject | embryoid bodies (EBs) | en_US |
dc.subject | IN-VITRO DIFFERENTIATION | en_US |
dc.subject | EMBRYOID BODIES | en_US |
dc.subject | HEMATOPOIETIC DEVELOPMENT | en_US |
dc.subject | ENDOTHELIAL-CELLS | en_US |
dc.subject | AGGREGATION | en_US |
dc.subject | CULTIVATION | en_US |
dc.subject | HOMOGENEITY | en_US |
dc.subject | SUSPENSION | en_US |
dc.subject | EFFICIENCY | en_US |
dc.subject | HYDROGELS | en_US |
dc.title | A microwell array system for stem cell culture | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/j.biomaterials.2007.10.030 | - |
dc.relation.journal | BIOMATERIALS | - |
dc.contributor.googleauthor | Moeller, Hannes-Christian | - |
dc.contributor.googleauthor | Mian, Matthew K. | - |
dc.contributor.googleauthor | Shrivastava, Shamit | - |
dc.contributor.googleauthor | Chung, Bong Geun | - |
dc.contributor.googleauthor | Khademhosseini, Ali | - |
dc.relation.code | 2008201314 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF ENGINEERING SCIENCES[E] | - |
dc.sector.department | DEPARTMENT OF BIONANO ENGINEERING | - |
dc.identifier.pid | bchung | - |
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